FastQCFastQC Report
Thu 4 Jun 2026
659_cAa-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_cAa-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20937
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC73

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218410.431293881644935No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC4352.077661556096862No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA3471.657353011415198No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC3451.6478005444906147No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3431.6382480775660315No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3281.566604575631657No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2621.251373167120409No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC1210.577924248937288No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1140.5444906147012466No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC1120.5349381477766633No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC1100.52538568085208No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC990.4728471127668721No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC950.4537421789177055No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG880.42030854468166406No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC810.38687491044562256No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC770.36776997659645605No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC670.32000764197353965No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC650.31045517504895637No Hit
GCGGCGGCAGCAGCAGATCACGGGAGTGCTGCAGCAGCCTCCACAGCAGC640.30567894158666475No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG630.3009027081243731No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG620.2961264746620815No Hit
CCGCCCCCCGGCCCCGCGTCC610.29135024119978986No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG580.2770215408129149No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC560.26746907388833163No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG540.2579166069637484No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT520.24836414003916513No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC500.23881167311458185No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG480.22925920618999857No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC470.22448297272770693No Hit
CCATCTTTCGGGTCCGCCCCCGGGGC450.21493050580312367No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC430.2053780388785404No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGT390.18627310502937383No Hit
GGACGCGGGGCCGGGGGGCGG380.1814968715670822No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC360.1719444046424989No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG360.1719444046424989No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA360.1719444046424989No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC360.1719444046424989No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC350.1671681711802073No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG350.1671681711802073No Hit
CCCCGTCCTCCCCCCTCCCCGGGG340.16239193771791566No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA340.16239193771791566No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG330.15761570425562402No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC330.15761570425562402No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG330.15761570425562402No Hit
CTCCGCCCCCCGGCCCCGCGTCC320.15283947079333238No Hit
CTCCAGTTCTGCTGCGACCAGTACAAGTTGGACACCAATTGCATCTCCTT310.14806323733104074No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA310.14806323733104074No Hit
GCCCCCCGGCCCCGCGTCC300.1432870038687491No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG290.13851077040645746No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG290.13851077040645746No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG290.13851077040645746No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG280.13373453694416582No Hit
GTTTGCAGGCAGAAATTTAGATAAGGATATCCTTAAACAGCTGTTTATAG280.13373453694416582No Hit
TGTTAAGATTGTATGTAATAAAGAACTGAATCCACTGCATTTCTTCCATG280.13373453694416582No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGT270.1289583034818742No Hit
CCCGCCCCCCGGCCCCGCGTCC270.1289583034818742No Hit
GACTGGAGTTCAGACGTGTGCTCTCCGATC270.1289583034818742Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGAAGGCGGGGACCGCTGGCAGGTGGAGGGGCAGTATGGTTCCAGGACCC270.1289583034818742No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTA260.12418207001958256No Hit
GTGCTGCTGCCGGGGGGGCC260.12418207001958256No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC250.11940583655729092No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCC250.11940583655729092No Hit
CCCGGGCCTGCAAGATGAGGGTCCCGCAGAAGAGTGTGTGACGCCTATGA250.11940583655729092No Hit
GACTGGAGTTCAGACGTGTGCTCTTCCGATC250.11940583655729092Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC240.11462960309499928No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGC240.11462960309499928No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG240.11462960309499928No Hit
CTTCGCCCCCCGGCCCCGCGTC240.11462960309499928No Hit
CCTGTTGCTACCCTGCCCCACACTATGCTCCAGCGTGCCAGGACCAGGAG230.10985336963270766No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG230.10985336963270766No Hit
GTGCTGCTGCCGGGGGGGC230.10985336963270766No Hit
CTTCGCGCCCCCCGTTCCTCC230.10985336963270766No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC230.10985336963270766No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA220.10507713617041602No Hit
CGCCCCCCGGCCCCGCGTCC210.10030090270812438No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG210.10030090270812438No Hit
GCTGCTGCCGGGGGGGCC210.10030090270812438No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC210.10030090270812438No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG210.10030090270812438No Hit
CCGGCCTCTTGGAGAGAAGGCCGCGGGGCCTCCAGCAGTGTGCACTCGGG210.10030090270812438No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC210.10030090270812438No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC210.10030090270812438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)