FastQCFastQC Report
Thu 4 Jun 2026
659_cq5-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_cq5-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34213
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC11303.302838102475667No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC10813.159617689182474No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC7272.124923274778593No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC5571.6280361266185368No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA5441.5900388741121798No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3330.9733142372782275No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3090.9031654634203372No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGGG3020.8827054043784527TruSeq Adapter, Index 4 (96% over 30bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2750.8037880337883261No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2730.7979423026335019No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2730.7979423026335019No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2220.648876158185485No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG2100.6138017712565399No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC2070.6050331745243036No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2050.5991874433694794No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2010.587495981059831No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1940.5670359220179464No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1870.5465758629760618No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1760.5144243416245287No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG1640.47934995469558356No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAG1440.4208926431473417No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGAAC1390.4062783152602812No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG1290.3770496594861602No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1280.37412679390874815No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG1270.37120392833133603No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1260.36828106275392397No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGAAC1250.36535819717651186No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC1220.35658960044427557No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1160.339052406979803No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1110.3244380790927425No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1020.29813228889603366No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA1010.29520942331862154No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1010.29520942331862154No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC990.2893636921637974No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGGA910.2659807675445007TruSeq Adapter, Index 4 (96% over 30bp)
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC900.2630579019670885No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC890.26013503638967644No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC820.23967497734779178No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGT790.23090638061555546No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG770.22506064946073132No Hit
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC730.21336918715108294No Hit
ACGGGAAGGCCCGGTGGGGAA710.2075234559962587No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC690.20167772484143454No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC640.18706339695437407No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC620.18121766579954987No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC600.1753719346447257No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC600.1753719346447257No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC600.1753719346447257No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC580.1695262034899015No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC570.1666033379124894No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC560.16368047233507732No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC530.154911875602841No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACTGCTTCCAAGATCTGGGG510.14906614444801683Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC500.14614327887060474No Hit
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGG490.14322041329319263TruSeq Adapter, Index 11 (96% over 33bp)
GCCCCGGGGGCGGACCCGAAAGATGG470.13737468213836845No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT460.13445181656095637No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC450.13152895098354425No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC450.13152895098354425No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC440.12860608540613216No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG430.12568321982872008No Hit
AGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC420.12276035425130799No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACTGCTTCCAAGATCTGGGG420.12276035425130799Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGAAC410.11983748867389589No Hit
CCCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC410.11983748867389589No Hit
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC410.11983748867389589No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGTG400.1169146230964838TruSeq Adapter, Index 4 (96% over 30bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC400.1169146230964838No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGC390.1139917575190717No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG390.1139917575190717No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC380.11106889194165961No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA370.1081460263642475No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGG370.1081460263642475TruSeq Adapter, Index 4 (96% over 31bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGT360.10522316078683543No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGT360.10522316078683543No Hit
GGACGCGGGGCCGGGGGGCGGG350.10230029520942331No Hit
GCTGCTGCCGGGGGGGCTGTAAAACTCGGGGGGCCCGG350.10230029520942331No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)