FastQCFastQC Report
Thu 4 Jun 2026
659_cuO-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_cuO-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22319
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC70

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12675.676777633406515No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA9644.3191899278641515No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC9244.139970428782651No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3401.5233657421927507No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC3061.3710291679734756No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2961.3262242932031005No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2200.9857072449482503No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2090.9364218827008379No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1690.7572023836193378No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1660.7437609211882252No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1330.5959048344459877No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG1330.5959048344459877No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC1270.5690219095837626No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCG1140.5107755723822752No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1090.4883731349970877No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC1020.45700972265782513No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG1000.44804874770375014No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG930.41668533536448765No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC890.39876338545633766No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG860.38532192302522517No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG840.3763609480711501No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC820.3673999731170751No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC820.3673999731170751No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG800.3584389981630001No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC790.3539585106859626No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC730.32707558582373764No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGC720.32259509834670014No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC690.30915363591558764No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC670.3001926609615126No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG650.2912316860074376No Hit
GCTGCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC630.2822707110533626No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC620.2777902235763251No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG610.2733097360992876No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG600.2688292486222501No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG580.2598682736681751No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC570.2553877861911376No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGC550.2464268112370626No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC530.2374658362829876No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGT520.2329853488059501No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC510.22850486132891257No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC500.22402437385187507No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC490.21954388637483757No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG480.2150633988978001No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG460.20610242394372508No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGG460.20610242394372508No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG440.19714144898965008No Hit
GCCGGGGGGGCTGTAACACTCGGGGGTTCC440.19714144898965008No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC430.19266096151261258No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC420.18818047403557506No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG420.18818047403557506No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCGG410.18369998655853756No Hit
GCTGCCGGGGGGGCTGTAACACTCGGG390.17473901160446256No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC380.17025852412742506No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC380.17025852412742506No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA370.16577803665038757No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG360.16129754917335007No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCA360.16129754917335007No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG350.15681706169631257No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGC350.15681706169631257No Hit
GCTGCCGGGGGGGCTGTAACACTCGG350.15681706169631257No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC340.15233657421927507No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC340.15233657421927507No Hit
GCTGCTCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC330.14785608674223755No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC320.14337559926520005No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG320.14337559926520005No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCG320.14337559926520005No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG310.13889511178816255No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC310.13889511178816255No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCA300.13441462431112505No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG280.12545364935705006No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGATGCGTG280.12545364935705006No Hit
TTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC270.12097316188001254No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG270.12097316188001254No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC260.11649267440297505No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA260.11649267440297505No Hit
GCTGCTGCCGGGGGGGCTGTAACACTC260.11649267440297505No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT250.11201218692593753No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG240.10753169944890005No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG240.10753169944890005No Hit
TTCCCCACCGGGCCTTCCCGT240.10753169944890005No Hit
CCGACACCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC240.10753169944890005No Hit
CAAGGTGTCTGCGGCCCTCACCCCCAGTCCCTGTCCCCACTCACCCAGCA240.10753169944890005No Hit
GCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC240.10753169944890005No Hit
CGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC240.10753169944890005No Hit
CATGGACAATGAAGAAAAAAGTGGGTAAAAAGTACATCAAAGCCCTTTCT230.10305121197186254No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC230.10305121197186254No Hit
CCGTGTCCTGAGCTCACCGGTTCTCCGGGGATTCCGAGGCCTAGCTGCTG230.10305121197186254No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGT230.10305121197186254No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC230.10305121197186254No Hit
GCTCACGGCAGTGGGCTGCAGGCTGTCCTGGCCGGGGGAGGTGTAGAGGC230.10305121197186254No Hit
CCGCCCCCCGGCCCCGCGTCC230.10305121197186254No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)