FastQCFastQC Report
Thu 4 Jun 2026
659_cuO-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_cuO-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22319
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA7703.449975357318876No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT6382.858551010349926No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4852.173036426363188No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG4592.056543751960213No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC3441.5412876921009007No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3021.3531072180653254No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2931.312782830771988No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC2100.9409023701778754No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1710.7661633585734128No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1700.7616828710963753No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1370.6138267843541377No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1360.6093462968771003No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTAGTA1350.6048658094000627TruSeq Adapter, Index 3 (96% over 30bp)
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1260.5645414221067252No Hit
CGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1140.5107755723822752No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1070.4794121600430127No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC1000.44804874770375014No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC990.44356826022671264No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC910.40772436041041266No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC910.40772436041041266No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC850.38084143554818767No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC830.3718804605941126No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC820.3673999731170751No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC810.3629194856400376No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC800.3584389981630001No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC770.34499753573188763No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG740.33155607330077513No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA730.32707558582373764No Hit
GCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC710.3181146108696626No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC700.31363412339262514No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC660.2957121734844751No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC640.2867511985304001No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT630.2822707110533626No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG620.2777902235763251No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG620.2777902235763251No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC620.2777902235763251No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGCAGC610.2733097360992876No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC600.2688292486222501No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGC570.2553877861911376No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCCC560.2509072987141001No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC550.2464268112370626No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG510.22850486132891257No Hit
ACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC510.22850486132891257No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG500.22402437385187507No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG490.21954388637483757No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC490.21954388637483757No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC480.2150633988978001No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCAC470.21058291142076255No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC460.20610242394372508No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGC450.20162193646668758No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC420.18818047403557506No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC410.18369998655853756No Hit
CCGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC410.18369998655853756No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTGGTA410.18369998655853756TruSeq Adapter, Index 3 (96% over 30bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC400.17921949908150006No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGC400.17921949908150006No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG380.17025852412742506No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG380.17025852412742506No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG360.16129754917335007No Hit
TGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC360.16129754917335007No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC350.15681706169631257No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTAGTG340.15233657421927507TruSeq Adapter, Index 3 (96% over 30bp)
GCCGGGGGGGCTGTAACACTCGGGGGGC340.15233657421927507No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG330.14785608674223755No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG330.14785608674223755No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC320.14337559926520005No Hit
CCGAGTGTTACAGCCCCCCCGGCAGC320.14337559926520005No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC320.14337559926520005No Hit
TGCCGGGGGGGCTGTAACACTCGGGGGGCC320.14337559926520005No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC310.13889511178816255No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTGGTG310.13889511178816255TruSeq Adapter, Index 3 (96% over 30bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGAGCAGC310.13889511178816255No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC300.13441462431112505No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC290.12993413683408755No Hit
CGAGTGTTACAGCCCCCCCGGCAGCAGC290.12993413683408755No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTGGGG280.12545364935705006TruSeq Adapter, Index 3 (96% over 30bp)
GAGGAGCCCTTCGCACTGGGGGCCGAGGCCTCCAACTTCCAGCAGGAGCC270.12097316188001254No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC270.12097316188001254No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG270.12097316188001254No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG260.11649267440297505No Hit
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTAG260.11649267440297505TruSeq Adapter, Index 1 (96% over 32bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTAG260.11649267440297505TruSeq Adapter, Index 3 (96% over 31bp)
GAGTGTTACAGCCCCCCCGGCAGCAGC260.11649267440297505No Hit
CACGCATCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC260.11649267440297505No Hit
GCTCACGGCAGTGGGCTGCAGGCTGTCCTGGCCGGGGGAGGTGTAGAGGC250.11201218692593753No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGC250.11201218692593753No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGTGTCGG250.11201218692593753No Hit
AGTCCAAGCCCTAGGCAGTGCCACCCTTACCCAGCCCAGCCTTGAAGACA250.11201218692593753No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC240.10753169944890005No Hit
GGACGCGGGGCCGGGGGGCGG240.10753169944890005No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG240.10753169944890005No Hit
ACGGGAAGGCCCGGTGGGGAA240.10753169944890005No Hit
ATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC230.10305121197186254No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACTTCTACCGGCATCTAG230.10305121197186254Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC230.10305121197186254No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC230.10305121197186254No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAG230.10305121197186254No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)