FastQCFastQC Report
Thu 4 Jun 2026
659_d0M-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_d0M-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39193
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC11712.9877784298216516No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC11302.883167912637461No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC7791.9875998264996302No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6021.5359885693873907No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC5711.4568928124920266No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTAGTA5001.2757380144413544TruSeq Adapter, Index 7 (96% over 30bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4521.1532671650549842No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3320.8470900415890593No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT3240.8266782333579976No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2900.7399280483759856No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2790.7118618120582757No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2330.5944939147296711No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA2220.5664276784119613No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG2100.5358099660653688No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2010.5128466818054244No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1990.507743729747659No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1980.5051922537187763No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1950.4975378256321282No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1950.4975378256321282No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1870.4771260174010665No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGA1860.4745745413721838No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA1860.4745745413721838No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1850.4720230653433011No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTGGTA1730.44140535299670863TruSeq Adapter, Index 7 (96% over 30bp)
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGA1680.42864797285229506No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1660.42354502079452966No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1550.39547878447681983No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG1490.3801699283035236No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1300.3316918837547521No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1290.32914040772586944No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1240.31638302758145587No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1210.30872859949480774No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1080.2755594111193326No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1020.26025055494603627No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC950.2423902227438573No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGT920.2347357946572092No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG870.22197841451279562No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC860.21942693848391293No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACCAATGCGTGTATCTAGTA820.2092210343683821Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACCAATGCGTGTATCTAGTA810.2066695583394994Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT780.19901513025285125No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC780.19901513025285125No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC750.19136070216620316No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC740.18880922613732043No Hit
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC730.18625775010843773No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC690.1760518459929069No Hit
ACGGGAAGGCCCGGTGGGGAAA690.1760518459929069No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC650.16584594187737606No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCA640.16329446584849336No Hit
GGACGCGGGGCCGGGGGGCGGA630.16074298981961066No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC630.16074298981961066No Hit
AACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC630.16074298981961066No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC630.16074298981961066No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC590.1505370857040798No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGA580.1479856096751971No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC570.14543413364631438No Hit
GATCGGAAGAGCAACGTCTGAACTCCAGTCACCAATGCGTGTATCTAGTA560.14288265761743169TruSeq Adapter, Index 7 (95% over 22bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG520.13267675350190083No Hit
GGAGGAACGGGGGGCGCGAAGA500.12757380144413544No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC500.12757380144413544No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC500.12757380144413544No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACCAATGCGTGTATCTAG490.12502232541525274Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTAG490.12502232541525274TruSeq Adapter, Index 7 (96% over 31bp)
GATCGGAAGAGCACAGTCTGAACTCCAGTCACCAATGCGTGTATCTAGTA490.12502232541525274No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC480.12247084938637001No Hit
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC480.12247084938637001No Hit
GATCGGAAGAGCACACGCTGAACTCCAGTCACCAATGCGTGTATCTAGTA470.1199193733574873No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGA450.11481642129972189No Hit
GATCGGAAGAGCACACGTCTGAACTCCGTCACCAATGCGTGTATCTAGTA450.11481642129972189Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC440.11226494527083919No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG440.11226494527083919No Hit
GATCGGAAGAGCACACTCTGAACTCCAGTCACCAATGCGTGTATCTAGTA430.10971346924195646TruSeq Adapter, Index 2 (95% over 21bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGA420.10716199321307376No Hit
CGGGGACTCGTGGGGTAACTTGTTCTTGGGAGCCAGCGGTATGGCGTCGG420.10716199321307376No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC420.10716199321307376No Hit
GATCGGAAGAGCACACGTCGAACTCCAGTCACCAATGCGTGTATCTAGTA410.10461051718419105No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC400.10205904115530835No Hit
CCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC400.10205904115530835No Hit
GGACGCGGGGCCGGGGGGCGGGA400.10205904115530835No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)