FastQCFastQC Report
Thu 4 Jun 2026
659_d0M-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_d0M-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39193
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC72

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30647.81772255249662No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC11833.0183961421682444No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC11112.8346898680886894No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC7912.0182175388462222No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC6621.689077131120353No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6301.6074298981961066No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5621.4339295282320823No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC3360.8572959457045901No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC2910.7424795244048682No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC2820.7195162401449238No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2010.5128466818054244No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC1990.507743729747659No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1960.500089301661011No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1960.500089301661011No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1960.500089301661011No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1950.4975378256321282No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1940.49498634960324545No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1870.4771260174010665No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1830.4669201132855356No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG1730.44140535299670863No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC1700.43375092491006045No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1670.4260964968234124No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1660.42354502079452966No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG1560.3980302605057025No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1310.33424335978363484No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC1260.3214859796392213No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1250.3189345036103386No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT1220.31128007552369047No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA1210.30872859949480774No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG1140.29086826729262877No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1020.26025055494603627No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG960.24494169877274002No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG860.21942693848391293No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA810.2066695583394994No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG780.19901513025285125No Hit
ACCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC780.19901513025285125No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG750.19136070216620316No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG750.19136070216620316No Hit
CCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACC750.19136070216620316No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGT730.18625775010843773No Hit
TTCCCCACCGGGCCTTCCCGT690.1760518459929069No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC690.1760518459929069No Hit
CCGCCCCCCGGCCCCGCGTCC680.17350036996402418No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA670.17094889393514145No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC650.16584594187737606No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG640.16329446584849336No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTT630.16074298981961066No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC630.16074298981961066No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG630.16074298981961066No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG590.1505370857040798No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC580.1479856096751971No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC560.14288265761743169No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC520.13267675350190083No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA510.13012527747301814No Hit
CTTCGCGCCCCCCGTTCCTCC500.12757380144413544No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC490.12502232541525274No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG490.12502232541525274No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC480.12247084938637001No Hit
CCCGCCCCCCGGCCCCGCGTCC470.1199193733574873No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTA460.1173678973286046No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG450.11481642129972189No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG450.11481642129972189No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG430.10971346924195646No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGTGGTGGTG430.10971346924195646No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA420.10716199321307376No Hit
TCAACCAGTGAACCTTTGGCATCATATCTTCCATGAATTTCTGCTCTCCA410.10461051718419105No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC410.10461051718419105No Hit
GCCCCCCGGCCCCGCGTCC400.10205904115530835No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)