FastQCFastQC Report
Thu 4 Jun 2026
659_d0V-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_d0V-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44782
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC14863.3182975302576927No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC14183.166450806127462No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC10302.3000312625608506No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA9022.014202134786298No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC8391.8735206109597606No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4911.0964226698226966No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3840.8574873833236568No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3770.8418561028984859No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3770.8418561028984859No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT3520.7860301013800187No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3450.7703988209548479No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC3290.7346701799830289No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2820.6297172971283105No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGA2780.6207851368853557No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC2590.5783573757313206No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2580.576124335670582No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTAGTA2580.576124335670582TruSeq Adapter, Index 5 (96% over 30bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG2390.5336965745165468No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1960.43767585190478314No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1900.4242776115403511No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1870.41757849135813496No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG1860.4153454512973963No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1770.3952480907507481No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA1640.3662185699611451No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTAGTG1640.3662185699611451TruSeq Adapter, Index 5 (96% over 30bp)
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1500.3349560091108034No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1490.33272296905006477No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1450.32379080880711003No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGA1420.31709168862489395No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1300.29029520789602964No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG1280.28582912777455227No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1210.27019784734938146No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1180.2634987271671654No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1060.23670224643830112No Hit
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1060.23670224643830112No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1040.2322361663168237No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG950.21213880577017555No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC940.2099057657094368No Hit
GGGGAGAGAGAGAGAGAGAGGGCGCGAAGA900.20097360546648207No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC900.20097360546648207No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGA820.18310928498057255No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC820.18310928498057255No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC800.17864320485909518No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTGGTG790.1764101647983565TruSeq Adapter, Index 5 (96% over 30bp)
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC780.1741771247376178No Hit
GGACGCGGGGCCGGGGGGCGGA770.1719440846768791No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC770.1719440846768791No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC760.16971104461614042No Hit
GCTGCTGCCGGGGGGGCCA750.1674780045554017No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC740.16524496449466303No Hit
GGACGCGGGGCCGGGGGGCGGGA710.15854584431244698No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGA680.1518467241302309No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC680.1518467241302309No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA680.1518467241302309No Hit
ACGGGAAGGCCCGGTGGGGAAA650.14514760394801482No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC630.14068152382653745No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGT600.13398240364432137No Hit
GCCCCCCCGGCAGCAGCACA590.1317493635835827No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCA590.1317493635835827No Hit
GGACGCGGGGCCGGGGGGCGGAGA580.129516323522844No Hit
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC580.129516323522844No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC570.1272832834621053No Hit
GGACGCGGGGCCGGGGGGCA570.1272832834621053No Hit
GGAGGAACGGGGGGCGCGAAGA540.12058416327988923No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCA540.12058416327988923No Hit
GGCCCCCCCGGCAGCAGCACA530.11835112321915056No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG520.11611808315841185No Hit
GCTGCTGCCGGGGGGCCCA510.11388504309767318No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCCCA510.11388504309767318No Hit
GCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC510.11388504309767318No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTAG510.11388504309767318TruSeq Adapter, Index 5 (96% over 31bp)
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC500.11165200303693448No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACACCAGGAATTATCTAGTA500.11165200303693448Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC500.11165200303693448No Hit
GGCCCCCCCGGCAGCAGCA480.1071859229154571No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGCA480.1071859229154571No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC480.1071859229154571No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC480.1071859229154571No Hit
GGAGGAACGGGGGGCGGGAAAGATGAAAGA480.1071859229154571No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGA480.1071859229154571No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG470.1049528828547184No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG470.1049528828547184No Hit
CCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC450.10048680273324104No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC450.10048680273324104No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)