FastQCFastQC Report
Thu 4 Jun 2026
659_dQk-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_dQk-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36535
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26267.187628301628575No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA8752.394963733406323No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC7832.143150403722458No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3931.0756808539756397No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC3370.9224031750376351No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2670.7308060763651293No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2440.6678527439441632No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2200.6021623101135897No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1920.5255234706445874No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1860.5091008621869441No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG1760.48172984809087177No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC1710.46804434104283565No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC1430.3914055015738333No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTCA1330.364034487477761No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1230.3366634733816888No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTCAGTA1220.3339263719720816No Hit
GCTGCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1090.29834405364718763No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1080.2956069522375804No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1050.2873956480087587No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCG1030.28192144518954426No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG970.2654988367319009No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG950.2600246339126865No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC900.24633912686465032No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTCAG890.24360202545504311No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC880.24086492404543589No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC870.23812782263582863No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC860.23539072122622146No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG850.2326536198166142No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC840.22991651840700697No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG780.21349390994936362No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG760.2080197071301492No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG740.2025455043109347No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC710.19433420008211305No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG710.19433420008211305No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG710.19433420008211305No Hit
GCCAAATTAAGGTGAAACCGCGAAAGGCTCATTAAATCAGTTATGGTTCC700.19159709867250582No Hit
CCTCACCAGGCCCGGACACCGGAAGGATTGACAGATTGAGAGCTCTTTCT690.1888599972628986No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC670.18338579444368414No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGC660.18064869303407693No Hit
GCGCGCTACACTGAAGGAATCAGCGTGTCCTCCCTGGCCGAGAGGCCCGG650.17791159162446968No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC640.17517449021486248No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTCAGTACAA630.17243738880525522No Hit
CTTAAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCCAAGGAGTG630.17243738880525522No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG620.16970028739564802No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC620.16970028739564802No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGC610.1669631859860408No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC580.1587518817572191No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC570.15601478034761188No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC540.1478034761187902No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC540.1478034761187902No Hit
GTTGAATGCACCGGTTCTCGTCCGATCACCGAAGTTAAGCAACGTCGGGC540.1478034761187902No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA540.1478034761187902No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGG530.145066374709183No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA520.14232927329957573No Hit
CGGACCGGTACGCGGTGGCGCTACGGCGTCGCCGATGTTGCTGGGAAGAT500.1368550704803613No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC490.13411796907075407No Hit
GCCGGGGGGGCTGTAACACTCGGGGGTTCC480.13138086766114684No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGC470.12864376625153962No Hit
GACTGGAGTTCAGACGTGTGCTCTCCGATC470.12864376625153962Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGCGTTGGTAACTTAAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCC470.12864376625153962No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC460.1259066648419324No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC460.1259066648419324No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTCAGT460.1259066648419324No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG460.1259066648419324No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCG460.1259066648419324No Hit
TTTCAAATGTCTGCCTTATCAACTGTCGATGGTAGGTTCTGCGCCTACCA460.1259066648419324No Hit
GCGAAAGGCTCATTAAATCAGTTATGGTTCCTTAGATCGTACCCACATTT460.1259066648419324No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG450.12316956343232516No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG450.12316956343232516No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCA430.11769536061311073No Hit
TGGTAACTTAAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCCAA420.11495825920350349No Hit
CCGCCCCCCGGCCCCGCGTCC420.11495825920350349No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG410.11222115779389627No Hit
CTTGGATCGTCGCAAGACGGACAGAAGCGAAAGCATTTGCCAAAAACGCT400.10948405638428904No Hit
CCTAAATCGACGCTTATGGGATACCATGAAAGGCGTTGGTAACTTAAGAC400.10948405638428904No Hit
GTTCGCTCGTCGTCGTACATTTTGGTGACTCTGAATAACTTTTTGCTGAT400.10948405638428904No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG400.10948405638428904No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA390.10674695497468181No Hit
GCACGGTCTTGCACCGGCGACGCATCTTTCAAATGTCTGCCTTATCAACT380.1040098535650746No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCGG380.1040098535650746No Hit
CACGTTGAATGCACCGGTTCTCGTCCGATCACCGAAGTTAAGCAACGTCG380.1040098535650746No Hit
GCACCGGCGACGCATCTTTCAAATGTCTGCCTTATCAACTGTCGATGGTA380.1040098535650746No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA370.10127275215546735No Hit
GCTGCCGGGGGGGCTGTAACACTCGGG370.10127275215546735No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)