FastQCFastQC Report
Thu 4 Jun 2026
659_dQk-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_dQk-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36535
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT6391.7490078007390175No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6211.6997399753660873No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGGG4481.2262214315040372TruSeq Adapter, Index 13 (96% over 30bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4341.187902011769536No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC3981.089366361023676No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3530.9661967975913507No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3330.9114547693992062No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2560.7006979608594499No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC2470.6760640481729848No Hit
GCGCCATCCATTTTCAGGGCTAGTAACTTCGGCAGGTGAGTTGTTACACA2430.6651156425345559No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1920.5255234706445874No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1900.520049267825373No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1730.4735185438620501No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC1710.46804434104283565No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1600.43793622553715617No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGC1380.3777199945257972No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1240.33940057479129604No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGTG1100.30108115505679484TruSeq Adapter, Index 13 (96% over 30bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1090.29834405364718763No Hit
CGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1030.28192144518954426No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGCAGC1030.28192144518954426No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1030.28192144518954426No Hit
CGCGGTTTCACCTTAATTTGGCTTGTACTGAGACATGCATGGCTTAATCT1000.2737101409607226No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC980.26823593814150815No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC970.2654988367319009No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC920.2518133296838648No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC890.24360202545504311No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC880.24086492404543589No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC870.23812782263582863No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG860.23539072122622146No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC850.2326536198166142No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA830.22717941699739977No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC810.22170521417818528No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGGTCATCTTCCCAGCAACATCG800.21896811276857808No Hit
ACAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACTCGCGCTTACTGG780.21349390994936362No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG780.21349390994936362No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC760.2080197071301492No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT750.20528260572054194No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC740.2025455043109347No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC700.19159709867250582No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC700.19159709867250582No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC690.1888599972628986No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGG680.18612289585329136TruSeq Adapter, Index 13 (96% over 31bp)
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC670.18338579444368414No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCCC670.18338579444368414No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC660.18064869303407693No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGGTCATCTTCCCAGCAAC650.17791159162446968No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG640.17517449021486248No Hit
TTTCGCGGTTTCACCTTAATTTGGCTTGTACTGAGACATGCATGGCTTAA640.17517449021486248No Hit
GCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC620.16970028739564802No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACAGAGTATGTGATCTGGGG610.1669631859860408Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGG590.16148898316682633TruSeq Adapter, Index 5 (97% over 34bp)
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC590.16148898316682633No Hit
CGAAAATACGCCTAGTTAGCAGGCTAGAGTCTCGTTCGTTATCGGAATTA590.16148898316682633No Hit
GTACAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACTCGCGCTTACT590.16148898316682633No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG580.1587518817572191No Hit
CCCGTTACCCGTTACAACCATGGTAGGCGCAGAACCTACCATCGACAGTT580.1587518817572191No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG570.15601478034761188No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC570.15601478034761188No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC540.1478034761187902No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCGC530.145066374709183No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCAC530.145066374709183No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACAGAGTATGTGATCTGG530.145066374709183Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGG530.145066374709183No Hit
AGACAATTCCGAGTTCCGACATCGAACTAAGATGCACCCATGATCGACCG510.13959217188996853No Hit
GCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAAATGTGGGTAC500.1368550704803613No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGC500.1368550704803613No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG490.13411796907075407No Hit
CCCGTTACAACCATGGTAGGCGCAGAACCTACCATCGACAGTTGATAAGG490.13411796907075407No Hit
GCTCAATCTCGTGCGGCTAGAAGCCGCCTGTCCCTCTAAGAAGAATTGAT480.13138086766114684No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGC480.13138086766114684No Hit
CCATTTTCAGGGCTAGTAACTTCGGCAGGTGAGTTGTTACACACTCCTTG480.13138086766114684No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTC470.12864376625153962Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TTTTACTTCCTCTAAATGATCAAGTTTGGTCATCTTCCCAGCAACATCGG470.12864376625153962No Hit
CGAGTGTTACAGCCCCCCCGGCAGCAGC470.12864376625153962No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC460.1259066648419324No Hit
TGCCGGGGGGGCTGTAACACTCGGGGGGCC450.12316956343232516No Hit
GTTACAACCATGGTAGGCGCAGAACCTACCATCGACAGTTGATAAGGCAG450.12316956343232516No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG440.12043246202271794No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC440.12043246202271794No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC440.12043246202271794No Hit
GATCGGAAGAGCACAGTCTGAACTCCAGTCACAGAGTATGTGATCTGGGG430.11769536061311073No Hit
CAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACTCGCGCTTACTGGG430.11769536061311073No Hit
GATCGGAAGAGCACACGTCGAACTCCAGTCACAGAGTATGTGATCTGGGG430.11769536061311073No Hit
ATTTGAAAGATGCGTCGCCGGTGCAAGACCGTGCGATCAGCAAAAAGTTA420.11495825920350349No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC420.11495825920350349No Hit
TGATAGTACGCCGACAGTAAAAACCGCGCGCGACACGACGCGAGAACGCC420.11495825920350349No Hit
ATCCATTTTCAGGGCTAGTAACTTCGGCAGGTGAGTTGTTACACACTCCT420.11495825920350349No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC420.11495825920350349No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA420.11495825920350349No Hit
GGACGCGGGGCCGGGGGGCGG410.11222115779389627No Hit
GTACGATCTAAGGAACCATAACTGATTTAATGAGCCTTTCGCGGTTTCAC410.11222115779389627No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACAGAGTATGTGATCTGGGG410.11222115779389627Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GACAATTCCGAGTTCCGACATCGAACTAAGATGCACCCATGATCGACCGC400.10948405638428904No Hit
GCCTTTCGCGGTTTCACCTTAATTTGGCTTGTACTGAGACATGCATGGCT380.1040098535650746No Hit
CTAGAATTACCACAGTTATCCAAGTAAATGTGGGTACGATCTAAGGAACC380.1040098535650746No Hit
CCGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC370.10127275215546735No Hit
CTTTAATCGTTGAACAAACGATTAAAG370.10127275215546735No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGC370.10127275215546735No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)