FastQCFastQC Report
Thu 4 Jun 2026
659_dkd-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_dkd-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28028
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC70

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC10793.849721706864564No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC10433.7212787212787215No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC7292.6009704581133155No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC5652.015841301555587No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3191.1381475667189953No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2931.045383188240331No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA2680.9561866704723847No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2360.8420151277294134No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTGGGG2300.8206079634651062TruSeq Adapter, Index 13 (96% over 30bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2240.7992007992007992No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2080.7421150278293136No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1980.7064364207221351No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG1960.6993006993006993No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1840.656486370772085No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1690.6029684601113172No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1660.5922648779791637No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1630.5815612958470101No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1580.5637219922934209No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1350.48166119594691026No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1310.46738975310403885No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC1290.4602540316826031No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1250.44598258883973174No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG1200.4281432852861425No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1080.38532895675752815No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG1050.37462537462537465No Hit
GCCCCGGGGGCGGACCCGAAAGATGG1050.37462537462537465No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1040.3710575139146568No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC1040.3710575139146568No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG1030.3674896532039389No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT1030.3674896532039389No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAG960.34251462822891393No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC880.3139717425431711No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT860.3068360211217354No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC850.30326816041101756No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC830.29613243898958186No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC820.292564578278864No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGT780.27829313543599254No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC770.27472527472527475No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC680.24261452832881406No Hit
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC670.23904666761809618No Hit
GGACGCGGGGCCGGGGGGCGGG610.21763950335378907No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC610.21763950335378907No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC610.21763950335378907No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC600.21407164264307124No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC570.20336806051091766No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC570.20336806051091766No Hit
CCCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC560.1998001998001998No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC560.1998001998001998No Hit
GGACGCGGGGCCGGGGGGCGG550.19623233908948193No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC530.18909661766804622No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC490.17482517482517482No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG490.17482517482517482No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC490.17482517482517482No Hit
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC480.17125731411445697No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTAGGG470.1676894534037391TruSeq Adapter, Index 13 (96% over 30bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG430.1534180105608677No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC430.1534180105608677No Hit
AACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC420.14985014985014986No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG410.146282289139432No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC390.13914656771799627No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC390.13914656771799627No Hit
CATCCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC390.13914656771799627No Hit
CGTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC350.12487512487512488No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC350.12487512487512488No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC350.12487512487512488No Hit
GGACGCGGGGCCGGGGGGCGGAG340.12130726416440703No Hit
CCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC340.12130726416440703No Hit
GGACGCGGGGCCGGGGGGC330.11773940345368916No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC330.11773940345368916No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACAGACGACAGAATCTGGGG320.11417154274297131Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGACGCGGGGCCGGGGGGCG320.11417154274297131No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG320.11417154274297131No Hit
CCGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC320.11417154274297131No Hit
ACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC310.11060368203225346No Hit
GGAGGAACGGGGGGCGCGAAG310.11060368203225346No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC300.10703582132153562No Hit
CGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC300.10703582132153562No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTGG290.10346796061081774TruSeq Adapter, Index 13 (96% over 31bp)
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGAGA290.10346796061081774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)