FastQCFastQC Report
Thu 4 Jun 2026
659_dwi-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_dwi-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81599
Total Bases3.6 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC29783.6495545288545204No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC26333.226755229843503No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC23272.8517506341989485No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC14811.8149732227110627No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA9271.1360433338643856No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC8020.9828551820487996No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC7250.8884912805303987No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTAGTA7030.8615301658108556TruSeq Adapter, Index 4 (96% over 30bp)
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC6210.7610387382198311No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC5650.6924104462064486No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC5130.6286841750511648No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5120.6274586698366401No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC4730.5796639664701773No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGA4660.5710854299685045No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT4650.5698599247539798No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC4530.5551538621796835No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC4310.5281927474601404No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3710.4546624345886592No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3700.45343692937413443No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3540.4338288459417395No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG3380.41422076250934453No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3380.41422076250934453No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2770.3394649444233385No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA2740.33578842877976445No Hit
GGACGCGGGGCCGGGGGGCGGA2710.3321119131361904No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG2670.32720989227809166No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2400.29412125148592505No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC2370.29044473584235103No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC2360.28921923062782634No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG2280.2794151889116288No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGA2280.2794151889116288No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC2250.27573867326805473No Hit
GCCCCGGGGGCGGACCCGAAAGATGGA2170.26593463155185726No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2150.2634836211228079No Hit
GGGGAGAGAGAGAGAGAGAGGGCGCGAAGA2040.2500030637630363No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1990.2438755376904129No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG1970.2414245272613635No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGCA1850.22671846468706722No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1830.22426745425801786No Hit
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1810.22181644382896848No Hit
CCGCCCCCCGGCCCCGCGTCCA1680.20588487604014755No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1650.20220836039657347No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1580.1936298238949007No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTGGTA1570.19240431868037597TruSeq Adapter, Index 4 (96% over 30bp)
GGACGCGGGGCCGGGGGGCGGGA1530.18750229782227723No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1510.18505128739322788No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1460.17892376132060442No Hit
GGACGCGGGGCCGGGGGGCCCA1450.17769825610607973No Hit
GGACGCGGGGCCGGGGGGCGGAGA1370.16789421438988222No Hit
CCCCGGGGAGGGGGGAGGACGGGGA1360.16666870917535756No Hit
GCTGCTGCCGGGGGGGCCA1350.16544320396083284No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1280.15686466745916003No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC1260.15441365703011067No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1250.15318815181558598No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC1200.14706062574296253No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1190.14583512052843786No Hit
AGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1180.14460961531391317No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1160.1421586048848638No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC1140.1397075944558144No Hit
CGCCCCCCGGCCCCGCGTCCA1130.1384820892412897No Hit
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1120.13725658402676502No Hit
GGACGCGGGGCCGGGGGGCA1100.13480557359771564No Hit
GGACGCGGGGCCGGGGGGCGAAGA1100.13480557359771564No Hit
GGAGGAACGGGGGGCGCGAAGA1080.13235456316866628No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGA1080.13235456316866628No Hit
GCCCCCCCGGCAGCAGCACA1030.12622703709604283No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACTGTGACGTATATCTAGTA1030.12622703709604283Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCTGCTGCCGGGGGGCCCA1020.12500153188151816No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC1010.12377602666699347No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG1010.12377602666699347No Hit
GGACGCGGGGCCGGGGGGCGG1010.12377602666699347No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACTGTGACGTATATCTAGTA1010.12377602666699347Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1000.12255052145246877No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCA970.11887400580889472No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTAG960.11764850059437003TruSeq Adapter, Index 4 (96% over 31bp)
ACGGGAAGGCCCGGTGGGGAAA960.11764850059437003No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCCCA950.11642299537984534No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGA950.11642299537984534No Hit
CGCGCCCTCTCTCTCTCTCTCTCCCCCGCA950.11642299537984534No Hit
GGCCCCCCCGGCAGCAGCA950.11642299537984534No Hit
GGCCCCCCCGGCAGCAGCACA940.11519749016532066No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG920.11274647973627129No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG910.11152097452174659No Hit
AACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC910.11152097452174659No Hit
GGACGCGGGGCCGGGGGGCCA900.1102954693072219No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC890.10906996409269722No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCA890.10906996409269722No Hit
CCCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC850.10416794323459845No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC840.10294243802007377No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCCGCA820.1004914275910244No Hit
CCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC820.1004914275910244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)