FastQCFastQC Report
Thu 4 Jun 2026
659_dwi-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_dwi-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81599
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC73

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39814.878736259022782No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC29913.665486096643341No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC26093.1973431046949106No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC23372.8640056863441954No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC14681.7990416549222417No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC10301.2622703709604284No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA8821.0808955992107747No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC8090.9914337185504725No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC7260.8897167857449234No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC6310.773293790365078No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC5740.7034399931371709No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC5140.6299096802656895No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC4710.577212956041128No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC4650.5698599247539798No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC4540.5563793673942082No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC4330.5306437578891898No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG3770.4620154658758073No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG3740.45833895023223326No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG3520.4313778355126901No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3330.4080932364367211No Hit
CCGCCCCCCGGCCCCGCGTCC3000.3676515643574063No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG2740.33578842877976445No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG2630.3223078714199929No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2480.3039252932021226No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC2420.29657226191497443No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG2420.29657226191497443No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG2350.28799372541330165No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA2340.2867682201987769No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2250.27573867326805473No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC2240.27451316805353004No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT2180.26716013676638195No Hit
CCATCTTTCGGGTCCGCCCCCGGGGC2150.2634836211228079No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2100.2573560950501844No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC2080.2549050846211351No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG2000.24510104290493753No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC1900.23284599075969067No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1870.22916947511611663No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGT1820.22304194904349317No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1760.21568891775634505No Hit
GGACGCGGGGCCGGGGGGCGG1670.20465937082562285No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG1640.2009828551820488No Hit
CCCGCCCCCCGGCCCCGCGTCC1640.2009828551820488No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG1590.19485532910942535No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG1530.18750229782227723No Hit
GCCCCCCGGCCCCGCGTCC1470.1801492665351291No Hit
CCCCGTCCTCCCCCCTCCCCGGGG1430.17524724567703037No Hit
GGGCCCCCCGGCCCCGCGTCC1430.17524724567703037No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA1420.17402174046250565No Hit
CTCCGCCCCCCGGCCCCGCGTCC1360.16666870917535756No Hit
GGCCCCCCCGGCAGCAGC1330.16299219353178349No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1260.15441365703011067No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC1250.15318815181558598No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1250.15318815181558598No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC1230.1507371413865366No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1190.14583512052843786No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1190.14583512052843786No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1190.14583512052843786No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCT1160.1421586048848638No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1140.1397075944558144No Hit
GGACGCGGGGCCGGGGGGCG1140.1397075944558144No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTA1130.1384820892412897No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG1110.13603107881224036No Hit
CTTCGCCCCCCGGCCCCGCGTCC1110.13603107881224036No Hit
CGCCCCCCGGCCCCGCGTCC1070.1311290579541416No Hit
CTTCGCGCCCCCCGTTCCTCC1060.1299035527396169No Hit
GTGCTGCTGCCGGGGGGGC1040.12745254231056755No Hit
GGGCCCCCCGGCAGCAGC1020.12500153188151816No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1000.12255052145246877No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1000.12255052145246877No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC980.1200995110234194No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG980.1200995110234194No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG970.11887400580889472No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGC960.11764850059437003No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC960.11764850059437003No Hit
GCTGCTGCCGGGGGGGCC960.11764850059437003No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG950.11642299537984534No Hit
TTCCCCACCGGGCCTTCCCGT940.11519749016532066No Hit
GGCCCCCCGGCCCCGCGTCC940.11519749016532066No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC920.11274647973627129No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTT920.11274647973627129No Hit
GTGCTGCTGCCGGGGGGGCC900.1102954693072219No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCC880.10784445887817252No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG880.10784445887817252No Hit
CCCCCGGCCCCGCGTCC850.10416794323459845No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGGG850.10416794323459845No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGGGG850.10416794323459845No Hit
CTTCGCCCCCCGGCCCCGCGTC830.10171693280554908No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG820.1004914275910244No Hit
GCCCCCCCGGCAGCAGC820.1004914275910244No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)