FastQCFastQC Report
Thu 4 Jun 2026
659_e0S-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_e0S-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12222
Total Bases939 kbp
Sequences flagged as poor quality0
Sequence length15-151
%GC71

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140011.45475372279496No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC6064.958271968581247No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA4643.7964326624120437No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1751.43184421534937No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1291.055473735886107No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1110.9081983308787434No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC890.7281950580919653No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC760.6218294878088693No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC700.572737686139748No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC640.5236458844706267No Hit
GGTCTCTCTTGAGCTCTCCTTGCGTTGCTCGCCTGTCTGTGTCTTCTGCG640.5236458844706267No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC610.49909998363606606No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC520.4254622811323842No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC470.38455244640811653No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC460.37637047946326296No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC420.3436426116838488No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG400.3272786777941417No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG400.3272786777941417No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC360.29455081001472755No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC340.27818687612502047No Hit
GATAGGGTTTCTCTTATTCCTTTTGGGCTGGGTGGAATGATGGTTGCCCT330.2700049091801669No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG330.2700049091801669No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC320.2618229422353133No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC320.2618229422353133No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC310.2536409752904598No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA310.2536409752904598No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC290.23727704140075273No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG280.22909507445589922No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC270.22091310751104565No Hit
CCCAGATGTAGGCATTAACTCCCTGCAGAACTACAAGCTTAGCCCCAATG270.22091310751104565No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGC260.2127311405661921No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC260.2127311405661921No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG260.2127311405661921No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG250.2045491736213386No Hit
CTCCCCACCGCGCGGGAGCACACCAGCGTGGGCTTCCCCGGCGCGCACGC240.19636720667648502No Hit
GCCATTTTCGGATTCCAGGTAAAGCAGGCAATGAATTCCACTGTAAGCCA240.19636720667648502No Hit
GCCGGGGGGGCTGTAACACTCGGGGGTTCC240.19636720667648502No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC240.19636720667648502No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC230.18818523973163148No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG220.18000327278677794No Hit
GGCTAACAAAAGCTTTTAAAAAATTAACTTAATTACCAAGGTAATGGCAG220.18000327278677794No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC220.18000327278677794No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC200.16363933889707086No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC200.16363933889707086No Hit
GCCCCTCGTACCTTCTCCAATAGTCTTGATCTGAATAAACAGATGGTTGA200.16363933889707086No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC190.15545737195221732No Hit
GGCTTTTTAAAAATATCCTTCGCTAGATAAAAAATCACGAGTAGAAATAC190.15545737195221732No Hit
CTTACTGATTTGATTTGCTAATGAGTTTTTTCTTGATGTGGTAAAATGAA180.14727540500736377No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCG180.14727540500736377No Hit
CTTCGAGGCAACTGTGCAGTTTGGGACGGTGCGGGGCCCCTATATCCCGG180.14727540500736377No Hit
CGGAACTGGCCGAGGAAATCTGCTCCAACGCCATCCAGATCCACGGCGGC180.14727540500736377No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG170.13909343806251023No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG170.13909343806251023No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC170.13909343806251023No Hit
GCTGCTGCCGGGGGGGCTGTAACACTC170.13909343806251023No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170.13909343806251023No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG160.13091147111765666No Hit
TGGAGGGTCCCGCAGAAGAGTGTGTGACGCCTATGAGTTTCAGCACAATA160.13091147111765666No Hit
GGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGCGGGACCGA160.13091147111765666No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG160.13091147111765666No Hit
GTGGCCTTCCACCCGCGGTACCCACTCTTTGCGTCAGGCTCGGACGACGG160.13091147111765666No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCA160.13091147111765666No Hit
AGCCGAAGCGCACCACATCCTTCTTCAGCCGGCAGCTGTCCTTGGGCCAG160.13091147111765666No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG150.12272950417280314No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG150.12272950417280314No Hit
CAGGCAGGCCAAGAACTTGTGCAGATGAGAAGCAAAATGATTTCTAATGC150.12272950417280314No Hit
CTCTGCATTATCTGGCGAGTTGTCTGCTTCCCTACCTGGCTTACTGTCCG150.12272950417280314No Hit
GTCATCTGTTCCAACTCTTAAGACAGACATGGTATTATTTGTCTCTATTT150.12272950417280314No Hit
GACTGGAGTTCAGACGTGTGCTCTCCGATC150.12272950417280314Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTTTTTAGGGCTAAATTCTGCTTTGTGACTGTCATCGTTTTGCAGGCCAC150.12272950417280314No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC150.12272950417280314No Hit
GCTGCCGGGGGGGCTGTAACACTCGGG150.12272950417280314No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGC150.12272950417280314No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC150.12272950417280314No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG140.11454753722794961No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC140.11454753722794961No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC140.11454753722794961No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC140.11454753722794961No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGC140.11454753722794961No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAG140.11454753722794961No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGC140.11454753722794961No Hit
CCGAGTGTTACAGCCCCCCCGGCAGC140.11454753722794961No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCA140.11454753722794961No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC130.10636557028309605No Hit
GTTACAGCCCCCCCGGCAGCAGC130.10636557028309605No Hit
AAAGTGTAAAAATATTTTCTAATCACATATTTGGTAGGTGTTAATTTCCA130.10636557028309605No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG130.10636557028309605No Hit
GCTGCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC130.10636557028309605No Hit
GCTGCCGGGGGGGCTGTAACACTCGG130.10636557028309605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)