FastQCFastQC Report
Thu 4 Jun 2026
659_e0S-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_e0S-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12222
Total Bases921.5 kbp
Sequences flagged as poor quality0
Sequence length15-151
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA4543.7146129929635086No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT3052.4954999181803306No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2562.0945835378825066No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC2552.086401570937653No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTG1901.5545737195221732TruSeq Adapter, Index 6 (96% over 30bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1771.448208149239077No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTA1761.4400261822942235TruSeq Adapter, Index 6 (96% over 30bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1100.9000163639338896No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC910.7445589919816723No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC760.6218294878088693No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC710.5809196530846016No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC640.5236458844706267No Hit
GGGAAAGACACAGTGAGAGTGTGTCAAGAGGCAGGGAACCAAGAGCCCTT640.5236458844706267No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC600.49091801669121254No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC520.4254622811323842No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAG510.4172803141875307TruSeq Adapter, Index 6 (96% over 31bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC470.38455244640811653No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCCA450.3681885125184094No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC420.3436426116838488No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCA400.3272786777941417No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA370.3027327769595811No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGA360.29455081001472755No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG350.286368843069874No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC350.286368843069874No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG330.2700049091801669No Hit
AAAGTGTAAAAATATTTTCTAATCACATATTTGGTAGGTGTTAATTTCCA330.2700049091801669No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC330.2700049091801669No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGCA320.2618229422353133No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG290.23727704140075273No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA290.23727704140075273No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC280.22909507445589922No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACGCTACAGGTCATCTAGTG280.22909507445589922Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACGCTACAGGTCATCTAGTA280.22909507445589922Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CTTGGATGTGCAAGTTGCCAGAGTGGACAGGGTCGCCACCATCAGAGGCA270.22091310751104565No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACGCTACAGGTCATCTAGTG270.22091310751104565Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC270.22091310751104565No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCA270.22091310751104565No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGA260.2127311405661921No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACGCTACAGGTCATCTAGTA260.2127311405661921Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCCGGGGGGGCTGTAACACTCGGGGGGCCCA260.2127311405661921No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC250.2045491736213386No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC250.2045491736213386No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC250.2045491736213386No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCA250.2045491736213386No Hit
GGCAAAACTAATGGCACAAGGAAAAATTTCAAACCCTTAAGCCAATTTTT240.19636720667648502No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG240.19636720667648502No Hit
GCCTCCTGGTACTCACGCTGCTGCTGTATACGGCGCTGTGGTGGTCGGCA220.18000327278677794No Hit
AATTAAGATAAAAGGGTTTTTAAAAATTATCTTTCTGACAAAATCTGCGG220.18000327278677794No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC220.18000327278677794No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACGCTACAGGTCATCTAG210.1718213058419244Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGAATATTTTGCTGATATGGAAAGGCATCGCATCTTGTTTAGATATGCTG210.1718213058419244No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG210.1718213058419244No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGGG210.1718213058419244TruSeq Adapter, Index 6 (96% over 30bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGC200.16363933889707086No Hit
GATCGGAAGAGCACACGTCTGAACCTCCAGTCACGCTACAGGTCATCTAG190.15545737195221732Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GAGAAGGCAGGCCATGGATCCCAACCAGACTGGAAACCCCAAAGGCAGGA190.15545737195221732No Hit
CGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGG190.15545737195221732No Hit
CGAGTGTTACAGCCCCCCCGGCAGCAGCA190.15545737195221732No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG190.15545737195221732No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC190.15545737195221732No Hit
GTCGCTGTTTCGTACTCGGTCGATGCTGCGTACGACGTCCTACACGTGCC180.14727540500736377No Hit
CAGGCAGGCCAAGAACTTGTGCAGATGAGAAGCAAAATGATTTCTAATGC180.14727540500736377No Hit
GATCGGAAGAGCCACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTA180.14727540500736377TruSeq Adapter, Index 6 (95% over 24bp)
GTCATCTGTTCCAACTCTTAAGACAGACATGGTATTATTTGTCTCTATTT180.14727540500736377No Hit
GATCGGAAGAGCACACGTTCTGAACTCCAGTCACGCTACAGGTCATCTAG180.14727540500736377No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC180.14727540500736377No Hit
GAAACCTAAGGCAACAACAAAGACAAATGCCCTGCCAGAGAACAGCTTGG180.14727540500736377No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG180.14727540500736377No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC170.13909343806251023No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTGGTG170.13909343806251023TruSeq Adapter, Index 6 (96% over 30bp)
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC170.13909343806251023No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG160.13091147111765666No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCA160.13091147111765666Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC160.13091147111765666No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC160.13091147111765666No Hit
CCAGTGCTGAAGACGGCACTGCCGGAGGGGCTGGGGCCGTATTGTGCTGA160.13091147111765666No Hit
CTTCGAGGCAACTGTGCAGTTTGGGACGGTGCGGGGCCCCTATATCCCGG160.13091147111765666No Hit
GCTATCGAAATGCACATCAGAAAAATGGTCACCATCTTCACTTGTTAAGT160.13091147111765666No Hit
AACCTGCTGAGGCAGCCCTCCCCACGTGGTGACCCATGAGACTGTTGCAG160.13091147111765666No Hit
GAGTGTTACAGCCCCCCCGGCAGCAGCA160.13091147111765666No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC150.12272950417280314No Hit
GATCGGAAGAGCACCGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTG150.12272950417280314TruSeq Adapter, Index 6 (95% over 22bp)
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC150.12272950417280314No Hit
GATCGGAAGAGCACAGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTA150.12272950417280314No Hit
ACGGCCAGACACCACTGCCCTGGGGAGGGGGCCAGGCAGGGTCAGCACTC150.12272950417280314No Hit
TGAAATTCTCGCAGGTATTACCTGGCTTCCTCAAGAACTCTGCCCATGAC150.12272950417280314No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCA150.12272950417280314No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG150.12272950417280314No Hit
GCTGCCGGGGGGGCTGTAACACTCGGA150.12272950417280314No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGC150.12272950417280314No Hit
GATCGGAAGAGCACACGTCTGAACTCCATCACGCTACAGGTCATCTAGTA150.12272950417280314Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CTTACTGATTTGATTTGCTAATGAGTTTTTTCTTGATGTGGTAAAATGAA140.11454753722794961No Hit
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACGCTACAGGTCATCTAG140.11454753722794961Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC140.11454753722794961No Hit
GCCGGGGGGGCTGTAACACTCGGGGGTTCCA140.11454753722794961No Hit
CGTTACCTTTCTGCTGAGGAACTCCCTGACCAAGGAGGCGGCCACCTCCT140.11454753722794961No Hit
GATCGGAAGAGCACACTCTGAACTCCAGTCACGCTACAGGTCATCTAGTA140.11454753722794961No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC140.11454753722794961No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGA140.11454753722794961No Hit
TGCCGGGGGGGCTGTAACACTCGGGGGGCCA140.11454753722794961No Hit
CGGGGGGGCTGTAACACTCGGGGGGCCA140.11454753722794961No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC140.11454753722794961No Hit
GCTGCTGCCGGGGGGGCTGTAACA130.10636557028309605No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCA130.10636557028309605No Hit
GATCGGAAGAGCACCACGTCTGAACTCCAGTCACGCTACAGGTCATCTAG130.10636557028309605TruSeq Adapter, Index 6 (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTTCCAGTCACGCTACAGGTCATCTAG130.10636557028309605Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCATCACGCTACAGGTCATCTAGTG130.10636557028309605Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC130.10636557028309605No Hit
GATCGGAAGAGCACACCGTCTGAACTCCAGTCACGCTACAGGTCATCTAG130.10636557028309605TruSeq Adapter, Index 6 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)