FastQCFastQC Report
Thu 4 Jun 2026
659_eeB-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_eeB-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19166
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6613.4488156109777734No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT5162.692267557132422No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3982.0765939684858603No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3531.8418031931545447No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3011.5704894083272463No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC2881.5026609621204217No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2511.3096107690702286No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGGG2431.2678701867891056TruSeq Adapter, Index 7 (96% over 30bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC2041.064384848168632No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1991.0382969842429302No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1210.6313263070019827No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1130.5895857247208598No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGC1120.5843681519357196No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1060.5530627152248774No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC990.516539705728895No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC910.4747991234477721No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC890.4643639778774914No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC840.4382761139517896No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC800.41740582281122823No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC780.4069706772409475No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC770.40175310445580714No Hit
GGGGCTTCCTCAGCATGGTCTCAGGTTCCCAGGGGGGGCGCAGCAGGCGT740.3861003861003861No Hit
CGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC690.3600125221746844No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC660.3443598038192633No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC650.3391422310341229No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC600.31305436710842116No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC580.30261922153814047No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC570.2974016487530001No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG560.2921840759678598No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG550.28696650318271943No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC540.28174893039757903No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG530.2765313576124387No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC530.2765313576124387No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC530.2765313576124387No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG520.27131378482729834No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC510.266096212042158No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCCC510.266096212042158No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC500.26087863925701765No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG490.2556610664718773No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC490.2556610664718773No Hit
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC460.24000834811645622No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGG460.24000834811645622TruSeq Adapter, Index 7 (96% over 31bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC440.2295732025461755No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG440.2295732025461755No Hit
GCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC430.22435562976103515No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGC410.21392048419075446No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGGA410.21392048419075446TruSeq Adapter, Index 7 (96% over 30bp)
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC400.20870291140561412No Hit
GATCGGAAGAGCACACGTCTGAACTCCGTCACCACGTAAGAGATCTGGGG390.20348533862047374Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCTGCCGGGGGGGCTGTAACACTCGGGGGGC390.20348533862047374No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG380.1982677658353334No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA360.1878326202650527No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC360.1878326202650527No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCAC350.18261504747991236No Hit
GGCCTGGAGGCAGGGGTGGTCTCTCTTGAGCTCTCCTTGCGTTGCTCGCC350.18261504747991236No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC340.177397474694772No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC340.177397474694772No Hit
ACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC330.17217990190963164No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC320.16696232912449127No Hit
GGACGCGGGGCCGGGGGGCGG310.16174475633935093No Hit
CGAGTGTTACAGCCCCCCCGGCAGCAGC310.16174475633935093No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACCACGTAAGAGATCTGGGG310.16174475633935093Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC310.16174475633935093No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC300.15652718355421058No Hit
CACAGTGAGAGTGTGTCAAGAGGCAGGGAACCAAGAGCCCTTGGCGCAGA300.15652718355421058No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG290.15130961076907024No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG290.15130961076907024No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC280.1460920379839299No Hit
ACGGGAAGGCCCGGTGGGGAA280.1460920379839299No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC270.14087446519878952No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC270.14087446519878952No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGAGCAGC270.14087446519878952No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC270.14087446519878952No Hit
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGG260.13565689241364917TruSeq Adapter, Index 7 (96% over 31bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG260.13565689241364917No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGC250.13043931962850883No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC250.13043931962850883No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACCACGTAAGAGATCTGGGG250.13043931962850883Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC240.12522174684336845No Hit
CTTTAATCGTTGAACAAACGATTAAAG240.12522174684336845No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAG230.12000417405822811No Hit
GATCGGAAGAGCACACGTCGAACTCCAGTCACCACGTAAGAGATCTGGGG230.12000417405822811No Hit
CCGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC230.12000417405822811No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC230.12000417405822811No Hit
GAGTGTTACAGCCCCCCCGGCAGCAGC230.12000417405822811No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT230.12000417405822811No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG220.11478660127308775No Hit
GATCGGAAGAGCACACGTTGAACTCCAGTCACCACGTAAGAGATCTGGGG220.11478660127308775No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC220.11478660127308775No Hit
TGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC220.11478660127308775No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGC220.11478660127308775No Hit
GCTGCCGGGGGGGCTGTAACACTCGGG220.11478660127308775No Hit
CCGAGTGTTACAGCCCCCCCGGCAGC210.1095690284879474No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG210.1095690284879474No Hit
TGCCGGGGGGGCTGTAACACTCGGGGGGCC210.1095690284879474No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC210.1095690284879474No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC200.10435145570280706No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG200.10435145570280706No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCG200.10435145570280706No Hit
GAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC200.10435145570280706No Hit
GATCGGAAGAGCAACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGGG200.10435145570280706TruSeq Adapter, Index 7 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)