FastQCFastQC Report
Thu 4 Jun 2026
659_eeB-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_eeB-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19166
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC71

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14137.372430345403318No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC8284.320150266096212No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA7623.9757904622769487No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC2971.5496191171866849No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2851.4870082437650005No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2501.3043931962850883No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2041.064384848168632No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC2041.064384848168632No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2011.0487321298132108No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1210.6313263070019827No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG1140.5948032975060003No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC1120.5843681519357196No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1050.547845142439737No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG980.5113221329437546No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG910.4747991234477721No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC880.459146405092351No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC800.41740582281122823No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC790.4121882500260879No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG770.40175310445580714No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC770.40175310445580714No Hit
CAGCTCCCAGCCAGGCCTACCAGGCAGCCTCCCGCAGCCCCCACAGCCAC710.37044766774496507No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCG670.34957737660440363No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC650.3391422310341229No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC640.33392465824898254No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG570.2974016487530001No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC570.2974016487530001No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG560.2921840759678598No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG540.28174893039757903No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC540.28174893039757903No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG530.2765313576124387No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC530.2765313576124387No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC530.2765313576124387No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGC510.266096212042158No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC510.266096212042158No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC500.26087863925701765No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG500.26087863925701765No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC480.2504434936867369No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC480.2504434936867369No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTA450.23479077533131587No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC440.2295732025461755No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC430.22435562976103515No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGC430.22435562976103515No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG420.2191380569758948No Hit
GCCGGGGGGGCTGTAACACTCGGGGGTTCC410.21392048419075446No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGC400.20870291140561412No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA390.20348533862047374No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC390.20348533862047374No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG360.1878326202650527No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG350.18261504747991236No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGG340.177397474694772No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG340.177397474694772No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGT330.17217990190963164No Hit
CACAGTGAGAGTGTGTCAAGAGGCAGGGAACCAAGAGCCCTTGGCGCAGA330.17217990190963164No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG320.16696232912449127No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC320.16696232912449127No Hit
CCGCCCCCCGGCCCCGCGTCC320.16696232912449127No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC310.16174475633935093No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCG310.16174475633935093No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG300.15652718355421058No Hit
GGCCTGGAGGCAGGGGTGGTCTCTCTTGAGCTCTCCTTGCGTTGCTCGCC300.15652718355421058No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG290.15130961076907024No Hit
TTCCCCACCGGGCCTTCCCGT290.15130961076907024No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG280.1460920379839299No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG280.1460920379839299No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC270.14087446519878952No Hit
GCTGCTCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC270.14087446519878952No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA260.13565689241364917No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC260.13565689241364917No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG250.13043931962850883No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG250.13043931962850883No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC250.13043931962850883No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAG240.12522174684336845No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC240.12522174684336845No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA240.12522174684336845No Hit
CTTTAATCGTTTGTTCAACGATTAAAG230.12000417405822811No Hit
GCTGCTGCCGGGGGGGCTGTAACACTC230.12000417405822811No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGTTCC230.12000417405822811No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG230.12000417405822811No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCGG230.12000417405822811No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG220.11478660127308775No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCA220.11478660127308775No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCA220.11478660127308775No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTC210.1095690284879474No Hit
GCTGCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC210.1095690284879474No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG210.1095690284879474No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGC210.1095690284879474No Hit
CGAGTGTTACAGCCCCCCCGGCAGCAGC200.10435145570280706No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGC200.10435145570280706No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC200.10435145570280706No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)