FastQCFastQC Report
Thu 4 Jun 2026
659_egp-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_egp-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11970
Total Bases746.6 kbp
Sequences flagged as poor quality0
Sequence length15-151
%GC77

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151612.66499582289056No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4043.3751044277360065No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2512.0969089390142024No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2031.6959064327485378No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1321.1027568922305764No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1150.960735171261487No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1000.835421888053467No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC900.7518796992481203No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC760.6349206349206349No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC670.559732664995823No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC650.5430242272347535No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG580.48454469507101083No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG530.4427736006683375No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG510.4260651629072682No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC500.4177109440267335No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC490.40935672514619886No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC440.36758563074352546No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC440.36758563074352546No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG430.3592314118629908No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG420.3508771929824561No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC420.3508771929824561No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC410.3425229741019215No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC400.3341687552213868No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC400.3341687552213868No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG400.3341687552213868No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC400.3341687552213868No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC390.3258145363408521No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG380.31746031746031744No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC380.31746031746031744No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGC350.29239766081871343No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG350.29239766081871343No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC320.2673350041771094No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG310.2589807852965748No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC300.2506265664160401No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG300.2506265664160401No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG300.2506265664160401No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC300.2506265664160401No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC290.24227234753550542No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCG290.24227234753550542No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC280.23391812865497078No Hit
GCCGGGGGGGCTGTAACACTCGGGGGTTCC280.23391812865497078No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC280.23391812865497078No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGC270.2255639097744361No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG260.2172096908939014No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA260.2172096908939014No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC250.20885547201336674No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC250.20885547201336674No Hit
CGAGTGTTACAGCCCCCCCGGCAGCAGC250.20885547201336674No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGC250.20885547201336674No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG250.20885547201336674No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC240.20050125313283207No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGG240.20050125313283207No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCG230.19214703425229743No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG230.19214703425229743No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG230.19214703425229743No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC230.19214703425229743No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC220.18379281537176273No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG220.18379281537176273No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG220.18379281537176273No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG220.18379281537176273No Hit
GCTGCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC210.17543859649122806No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAG210.17543859649122806No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGC200.1670843776106934No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCA200.1670843776106934No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC190.15873015873015872No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC190.15873015873015872No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG180.15037593984962408No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC180.15037593984962408No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCGG180.15037593984962408No Hit
CCGCCCCCCGGCCCCGCGTCC180.15037593984962408No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG170.14202172096908938No Hit
TTCCCCACCGGGCCTTCCCGT170.14202172096908938No Hit
GCTGCTGCCGGGGGGGCTGTAACACTC160.1336675020885547No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG150.12531328320802004No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC150.12531328320802004No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGATGCGTG150.12531328320802004No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGTTCC150.12531328320802004No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC150.12531328320802004No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA150.12531328320802004No Hit
GCTGCTGCCGGGGGGGCTGTAACAC140.11695906432748539No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG140.11695906432748539No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC140.11695906432748539No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCA140.11695906432748539No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGC140.11695906432748539No Hit
GCTGCCGGGGGGGCTGTAACACTCGG130.1086048454469507No Hit
GCAGGAGTCTGAAGAGGAGGAAGTCCCCCAGGAGTCCTGGGATGAAGGTT130.1086048454469507No Hit
TCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC130.1086048454469507No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG130.1086048454469507No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG130.1086048454469507No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC130.1086048454469507No Hit
GCTGACCCACTCTCTGGGGGGCGGCACGGGGTCCGGGATGGGCACCCTGC120.10025062656641603No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAG120.10025062656641603No Hit
GCCGGGGGGGCTGTAACACTCGGGGGG120.10025062656641603No Hit
CCCACCCCCGCGGGGAATCCCC120.10025062656641603No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG120.10025062656641603No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT120.10025062656641603No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC120.10025062656641603No Hit
CTGGCTACAATGCTGCTTCTTCTCCTTCCTGGAGACCAAGGGGTCACCCT120.10025062656641603No Hit
CCGACACCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC120.10025062656641603No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGC120.10025062656641603No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG120.10025062656641603No Hit
GCTGCCGGGGGGGCTGTAACACTCGGG120.10025062656641603No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGC120.10025062656641603No Hit
CTTCGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC120.10025062656641603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)