FastQCFastQC Report
Thu 4 Jun 2026
659_egp-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_egp-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11970
Total Bases718.9 kbp
Sequences flagged as poor quality0
Sequence length15-151
%GC69

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA3112.5981620718462826No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC2071.7293233082706767No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1861.5538847117794485No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGTA1681.4035087719298245TruSeq Adapter, Index 6 (96% over 33bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT1621.3533834586466165No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1321.1027568922305764No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC1311.0944026733500418No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1160.9690893901420217No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1040.8688387635756056No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGTG930.7769423558897243TruSeq Adapter, Index 6 (96% over 33bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC850.7101086048454469No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC770.6432748538011697No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC670.559732664995823No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC640.5346700083542189No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC640.5346700083542189No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGGG560.46783625730994155TruSeq Adapter, Index 6 (96% over 33bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTGGTG530.4427736006683375TruSeq Adapter, Index 6 (96% over 33bp)
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC510.4260651629072682No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC490.40935672514619886No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTGGGG490.40935672514619886TruSeq Adapter, Index 6 (96% over 33bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC490.40935672514619886No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC440.36758563074352546No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC420.3508771929824561No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG420.3508771929824561No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC420.3508771929824561No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC420.3508771929824561No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG410.3425229741019215No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC410.3425229741019215No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG400.3341687552213868No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC400.3341687552213868No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG390.3258145363408521No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC390.3258145363408521No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC380.31746031746031744No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGGA360.30075187969924816TruSeq Adapter, Index 6 (96% over 33bp)
GCCGGGGGGGCTGTAACACTCGGGGGGCCC360.30075187969924816No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC360.30075187969924816No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC350.29239766081871343No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG330.2756892230576441No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTGGTA330.2756892230576441TruSeq Adapter, Index 6 (96% over 33bp)
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC320.2673350041771094No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAG310.2589807852965748TruSeq Adapter, Index 6 (97% over 34bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG300.2506265664160401No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC300.2506265664160401No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGC300.2506265664160401No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACGCCAAGTAACATCTAGTA300.2506265664160401Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC290.24227234753550542No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGC290.24227234753550542No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC290.24227234753550542No Hit
CGAGTGTTACAGCCCCCCCGGCAGCAGC280.23391812865497078No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC280.23391812865497078No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA270.2255639097744361No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGC270.2255639097744361No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG270.2255639097744361No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG260.2172096908939014No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACGCCAAGTAACATCTAG260.2172096908939014Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG260.2172096908939014No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG250.20885547201336674No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC250.20885547201336674No Hit
CGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC240.20050125313283207No Hit
GCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC240.20050125313283207No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG240.20050125313283207No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG230.19214703425229743No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG230.19214703425229743No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCAC230.19214703425229743No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC230.19214703425229743No Hit
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAG230.19214703425229743TruSeq Adapter, Index 6 (97% over 34bp)
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC230.19214703425229743No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC230.19214703425229743No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC220.18379281537176273No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGCAGC220.18379281537176273No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC220.18379281537176273No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCG220.18379281537176273No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGC210.17543859649122806No Hit
TGCCGGGGGGGCTGTAACACTCGGGGGGCC210.17543859649122806No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGC210.17543859649122806No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC210.17543859649122806No Hit
GATCGGAAGAGCACCGTCTGAACTCCAGTCACGCCAAGTAACATCTAGTA200.1670843776106934TruSeq Adapter, Index 6 (95% over 23bp)
CCGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC190.15873015873015872No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG190.15873015873015872No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG190.15873015873015872No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC180.15037593984962408No Hit
GATCGGAAGAGCACACGTCTGAACTTCCAGTCACGCCAAGTAACATCTAG180.15037593984962408Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAG180.15037593984962408No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC180.15037593984962408No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACGCCAAGTAACATCTAGTA180.15037593984962408Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGACGCGGGGCCGGGGGGCGG170.14202172096908938No Hit
GAGTGTTACAGCCCCCCCGGCAGCAGC160.1336675020885547No Hit
ACGGGAAGGCCCGGTGGGGAA160.1336675020885547No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAG150.12531328320802004No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC150.12531328320802004No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC150.12531328320802004No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC150.12531328320802004No Hit
GATCGGAAGAGCACACTCTGAACTCCAGTCACGCCAAGTAACATCTAGTA140.11695906432748539TruSeq Adapter, Index 6 (95% over 21bp)
CTGTTCCTCTAATGAGTGAAATGTGCTGCTGTAAGACTTGTACGAGGCCA140.11695906432748539No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGC140.11695906432748539No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC140.11695906432748539No Hit
TGCCGGGGGGGCTGTAACACTCGGGGGGCCC140.11695906432748539No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC140.11695906432748539No Hit
GGGCTCAGGGTCTCTGGGGAGCAATCCCTGACTTGGGGGCTGGGTGGCCC130.1086048454469507No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCCCGG130.1086048454469507No Hit
GATCGGAAGAGCACAGTCTGAACTCCAGTCACGCCAAGTAACATCTAGTA130.1086048454469507TruSeq Adapter, Index 6 (95% over 23bp)
CCCCCCGAGTGTTACAGCCCCCCCGGC130.1086048454469507No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAG130.1086048454469507No Hit
CCGAGTGTTACAGCCCCCCCGGCAGC130.1086048454469507No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC130.1086048454469507No Hit
GTGTTACAGCCCCCCCGGCAGCAGC130.1086048454469507No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC130.1086048454469507No Hit
GGGGATTCCCCGCGGGGGTGGG120.10025062656641603No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC120.10025062656641603No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC120.10025062656641603No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCGAAG120.10025062656641603No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG120.10025062656641603No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGC120.10025062656641603No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC120.10025062656641603No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGC120.10025062656641603No Hit
CACGCATCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC120.10025062656641603No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTGG120.10025062656641603TruSeq Adapter, Index 6 (97% over 34bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGTGTCGG120.10025062656641603No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGTTCC120.10025062656641603No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACGCCAAGTAACATCTAGTG120.10025062656641603Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)