FastQCFastQC Report
Thu 4 Jun 2026
NTC_0001_0001_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23560
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length31-151
%GC70

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13525.738539898132427No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC8713.696943972835314No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC7253.0772495755517824No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC6362.699490662139219No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4992.1179966044142615No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC4641.9694397283531409No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA3621.5365025466893039No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC2310.9804753820033955No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC2270.963497453310696No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC2200.9337860780984719No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1790.7597623089983022No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1690.7173174872665534No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1520.6451612903225806No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCCAGATCGGAAGAGCGTCGTGT1450.6154499151103565No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1340.568760611205433No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1230.5220713073005093No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG1090.4626485568760611No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1090.4626485568760611No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1010.42869269949066213No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG970.4117147707979626No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG940.398981324278438No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG850.36078098471986414No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAGATCGGAAGAGCGTCGTGT780.3310696095076401No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG780.3310696095076401No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC770.32682512733446517No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC760.3225806451612903No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGAGATCGGAAGAGCGTCGTGT740.31409168081494054No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG690.2928692699490662No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC690.2928692699490662No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG690.2928692699490662No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCAGATCGGAAGAGCGTCGTGTA670.2843803056027165Illumina Single End PCR Primer 1 (100% over 21bp)
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA650.2758913412563667No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC590.25042444821731746No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGT560.23769100169779284No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC560.23769100169779284No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA550.23344651952461798No Hit
CCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGA540.22920203735144312Illumina Single End PCR Primer 1 (100% over 29bp)
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG530.22495755517826826No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG510.2164685908319185No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT480.20373514431239387No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGAGATCGGAAGAGCGTCGTGTA460.19524617996604413Illumina Single End PCR Primer 1 (100% over 21bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG460.19524617996604413No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG450.19100169779286927No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC450.19100169779286927No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGAGATCGGAAGAGCGTCGTGTA400.1697792869269949Illumina Single End PCR Primer 1 (100% over 21bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG390.16553480475382004No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC390.16553480475382004No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG370.15704584040747027No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC370.15704584040747027No Hit
GTGCTGCTGCCGGGGGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT370.15704584040747027Illumina Single End PCR Primer 1 (100% over 31bp)
CCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCGTCGTGTAGGGAAAG360.15280135823429541Illumina Single End PCR Primer 1 (100% over 28bp)
GGGCCCCCCGGCAGCAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG360.15280135823429541Illumina Single End PCR Primer 1 (100% over 32bp)
TTCCCCACCGGGCCTTCCCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGA360.15280135823429541Illumina Single End PCR Primer 1 (100% over 29bp)
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA350.14855687606112053No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC340.14431239388794567No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG340.14431239388794567No Hit
GGCCCCCCCGGCAGCAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG340.14431239388794567Illumina Single End PCR Primer 1 (100% over 32bp)
GCGGGGGAGAGAGAGAGAGAGAGGGCGCGAGATCGGAAGAGCGTCGTGTA320.13582342954159593Illumina Single End PCR Primer 1 (100% over 21bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC320.13582342954159593No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGAGATCGGAAGAGCGTCGTGTA310.13157894736842105Illumina Single End PCR Primer 1 (100% over 21bp)
CTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCGTCGTGTAGGGAAA290.1230899830220713Illumina Single End PCR Primer 1 (100% over 27bp)
GCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT290.1230899830220713Illumina Single End PCR Primer 1 (100% over 31bp)
TTCCCCACCGGGCCTTCCCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAG280.11884550084889642Illumina Single End PCR Primer 1 (100% over 28bp)
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCACG280.11884550084889642No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG280.11884550084889642No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCA280.11884550084889642No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTA270.11460101867572156No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCG270.11460101867572156No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC260.11035653650254668No Hit
GCCCCCCCGGCAGCAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT260.11035653650254668Illumina Single End PCR Primer 1 (100% over 33bp)
GGACGCGGGGCCGGGGGGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGA260.11035653650254668Illumina Single End PCR Primer 1 (100% over 29bp)
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGGG260.11035653650254668No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTT250.10611205432937182No Hit
GCTGCTGCCGGGGGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT250.10611205432937182Illumina Single End PCR Primer 1 (100% over 33bp)
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG240.10186757215619693No Hit
GCTGCTGCCGGGGGGGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG240.10186757215619693Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)