FastQCFastQC Report
Thu 4 Jun 2026
NTC_0001_0001_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23560
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC8623.6587436332767407No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC7233.068760611205433No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC6422.7249575551782685No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC4681.9864176570458403No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA4561.935483870967742No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3071.303056027164686No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGGG2320.9847198641765704TruSeq Adapter, Index 13 (96% over 30bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2260.9592529711375213No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2240.9507640067911713No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2180.9252971137521222No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1790.7597623089983022No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1700.7215619694397283No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1690.7173174872665534No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT1530.6494057724957555No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1510.6409168081494058No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAG1480.6281833616298811No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1340.568760611205433No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1270.5390492359932089No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1160.4923599320882852No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1090.4626485568760611No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC990.4202037351443124No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC920.39049235993208825No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC840.3565365025466893No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG800.3395585738539898No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG780.3310696095076401No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC770.32682512733446517No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC770.32682512733446517No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC770.32682512733446517No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG700.29711375212224106No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC690.2928692699490662No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC680.28862478777589134No Hit
GGGGAGAGAGAGAGAGAGAGGGCGCGAAG670.2843803056027165No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC640.27164685908319186No Hit
GGACGCGGGGCCGGGGGGCGG590.25042444821731746No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG590.25042444821731746No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC590.25042444821731746No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC570.24193548387096775No Hit
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC550.23344651952461798No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC530.22495755517826826No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG520.22071307300509335No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC510.2164685908319185No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA490.20797962648556878No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC480.20373514431239387No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG470.199490662139219No Hit
ACGGGAAGGCCCGGTGGGGAA470.199490662139219No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC460.19524617996604413No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC460.19524617996604413No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC450.19100169779286927No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG400.1697792869269949No Hit
GCTGCTGCCGGGGGGCCC400.1697792869269949No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC400.1697792869269949No Hit
GGACGCGGGGCCGGGGGGCGGG390.16553480475382004No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACAGAAGATTGGATCTGGGG390.16553480475382004Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC380.16129032258064516No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG370.15704584040747027No Hit
GCCCCCCCGGCAGCAGCAC370.15704584040747027No Hit
GCTGCTGCCGGGGGGGCC360.15280135823429541No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGG360.15280135823429541TruSeq Adapter, Index 13 (96% over 31bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACAGAAGATTGGATCTGGGG350.14855687606112053Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC350.14855687606112053No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC350.14855687606112053No Hit
GCTGCTGCCGGGGGGGC340.14431239388794567No Hit
GGACGCGGGGCCGGGGGGC330.1400679117147708No Hit
CGCGCCCTCTCTCTCTCTCTCTCCCCCGC320.13582342954159593No Hit
GGACGCGGGGCCGGGGGGCG320.13582342954159593No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG320.13582342954159593No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC310.13157894736842105No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC310.13157894736842105No Hit
CACGGGAAGGCCCGGTGGGGAA290.1230899830220713No Hit
TGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC290.1230899830220713No Hit
GGACGCGGGGCCGGGGGGCGGAG290.1230899830220713No Hit
CGTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC280.11884550084889642No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGT270.11460101867572156No Hit
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC270.11460101867572156No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG270.11460101867572156No Hit
CGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC270.11460101867572156No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC270.11460101867572156No Hit
CCGCCCCCCGGCCCCGCGTCC270.11460101867572156No Hit
AACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC260.11035653650254668No Hit
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGG260.11035653650254668TruSeq Adapter, Index 2 (96% over 32bp)
GGCCCCCCCGGCAGCAGC260.11035653650254668No Hit
CCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC260.11035653650254668No Hit
GCCCCCCCGGCAGCAGC250.10611205432937182No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG250.10611205432937182No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC250.10611205432937182No Hit
GATCGGAAGAGCACACGCTGAACTCCAGTCACAGAAGATTGGATCTGGGG240.10186757215619693No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)