FastQCFastQC Report
Thu 4 Jun 2026
NTC_0001_0001_B23MHV2LT4_2_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23MHV2LT4_2_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244434
Total Bases35.4 Mbp
Sequences flagged as poor quality0
Sequence length31-151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGT13701956.05562237659245TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGT4048016.56070759386992TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTAGT84483.4561476717641573TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGG46341.8958082754444963TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTGGT36141.4785177184843352TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGG25981.0628635950808807TruSeq Adapter, Index 1 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTA19480.7969431421160722TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCATCATCTATCTAGT14340.5866614300792853TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTCATCATCTATCTAGT11470.46924732238559286TruSeq Adapter, Index 12 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTATG9140.3739250677074384TruSeq Adapter, Index 1 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCATCATCTATCTGGT8370.342423721740838TruSeq Adapter, Index 12 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA5360.21928209659867284No Hit
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTCATCATCTATCTGGT5270.2156001210960832TruSeq Adapter, Index 12 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGTA5000.20455419458831423TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCGAGTCACCTCATCATCTATCTAGT4860.19882667713984142TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGCTCATCATCTATCTAGT3690.1509609956061759TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCGAGTCACCTCATCATCTATCTGGT3660.14973367043864602TruSeq Adapter, Index 12 (97% over 35bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT3450.1411423942659368No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC3410.1395059607092303No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGCTCATCATCTATCTGGT3160.1292782509798146TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCATCATCTATCTGGG3150.12886914259063798TruSeq Adapter, Index 12 (97% over 35bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3060.1251871670880483No Hit
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTGGG3040.12436895030969505TruSeq Adapter, Index 12 (97% over 35bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3030.12395984192051843No Hit
GATCGGAAGAGCACACGTCTGAACTCTGGTCACCTCATCATCTATCTAGT2710.11086837346686632Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTG2450.10023155534827398TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)