[W::hts_idx_load3] The index file is older than the data file: 659_6i-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (9449283) splicing reads. 227641 splicing junctions. 178868 known splicing junctions. 48773 novel splicing junctions.
sampling 10% (18898566) splicing reads. 267778 splicing junctions. 190789 known splicing junctions. 76989 novel splicing junctions.
sampling 15% (28347849) splicing reads. 293880 splicing junctions. 196789 known splicing junctions. 97091 novel splicing junctions.
sampling 20% (37797132) splicing reads. 313172 splicing junctions. 200730 known splicing junctions. 112442 novel splicing junctions.
sampling 25% (47246415) splicing reads. 328109 splicing junctions. 203409 known splicing junctions. 124700 novel splicing junctions.
sampling 30% (56695698) splicing reads. 339942 splicing junctions. 205376 known splicing junctions. 134566 novel splicing junctions.
sampling 35% (66144980) splicing reads. 349687 splicing junctions. 206938 known splicing junctions. 142749 novel splicing junctions.
sampling 40% (75594264) splicing reads. 357701 splicing junctions. 208213 known splicing junctions. 149488 novel splicing junctions.
sampling 45% (85043547) splicing reads. 364298 splicing junctions. 209156 known splicing junctions. 155142 novel splicing junctions.
sampling 50% (94492830) splicing reads. 370077 splicing junctions. 209997 known splicing junctions. 160080 novel splicing junctions.
sampling 55% (103942113) splicing reads. 374876 splicing junctions. 210683 known splicing junctions. 164193 novel splicing junctions.
sampling 60% (113391396) splicing reads. 378967 splicing junctions. 211266 known splicing junctions. 167701 novel splicing junctions.
sampling 65% (122840679) splicing reads. 382370 splicing junctions. 211718 known splicing junctions. 170652 novel splicing junctions.
sampling 70% (132289961) splicing reads. 385388 splicing junctions. 212106 known splicing junctions. 173282 novel splicing junctions.
sampling 75% (141739245) splicing reads. 387780 splicing junctions. 212432 known splicing junctions. 175348 novel splicing junctions.
sampling 80% (151188528) splicing reads. 389807 splicing junctions. 212683 known splicing junctions. 177124 novel splicing junctions.
sampling 85% (160637811) splicing reads. 391338 splicing junctions. 212872 known splicing junctions. 178466 novel splicing junctions.
sampling 90% (170087094) splicing reads. 392650 splicing junctions. 213056 known splicing junctions. 179594 novel splicing junctions.
sampling 95% (179536377) splicing reads. 393541 splicing junctions. 213177 known splicing junctions. 180364 novel splicing junctions.
sampling 100% (188985660) splicing reads. 394061 splicing junctions. 213242 known splicing junctions. 180819 novel splicing junctions.