[W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (7168701) splicing reads. 183286 splicing junctions. 155362 known splicing junctions. 27924 novel splicing junctions.
sampling 10% (14337402) splicing reads. 199844 splicing junctions. 162579 known splicing junctions. 37265 novel splicing junctions.
sampling 15% (21506103) splicing reads. 207629 splicing junctions. 165407 known splicing junctions. 42222 novel splicing junctions.
sampling 20% (28674804) splicing reads. 212424 splicing junctions. 167013 known splicing junctions. 45411 novel splicing junctions.
sampling 25% (35843505) splicing reads. 215739 splicing junctions. 168013 known splicing junctions. 47726 novel splicing junctions.
sampling 30% (43012206) splicing reads. 218037 splicing junctions. 168673 known splicing junctions. 49364 novel splicing junctions.
sampling 35% (50180908) splicing reads. 219946 splicing junctions. 169213 known splicing junctions. 50733 novel splicing junctions.
sampling 40% (57349609) splicing reads. 221486 splicing junctions. 169594 known splicing junctions. 51892 novel splicing junctions.
sampling 45% (64518310) splicing reads. 222755 splicing junctions. 169888 known splicing junctions. 52867 novel splicing junctions.
sampling 50% (71687011) splicing reads. 223829 splicing junctions. 170133 known splicing junctions. 53696 novel splicing junctions.
sampling 55% (78855712) splicing reads. 224783 splicing junctions. 170351 known splicing junctions. 54432 novel splicing junctions.
sampling 60% (86024413) splicing reads. 225568 splicing junctions. 170512 known splicing junctions. 55056 novel splicing junctions.
sampling 65% (93193114) splicing reads. 226221 splicing junctions. 170661 known splicing junctions. 55560 novel splicing junctions.
sampling 70% (100361816) splicing reads. 226812 splicing junctions. 170772 known splicing junctions. 56040 novel splicing junctions.
sampling 75% (107530517) splicing reads. 227295 splicing junctions. 170860 known splicing junctions. 56435 novel splicing junctions.
sampling 80% (114699218) splicing reads. 227677 splicing junctions. 170928 known splicing junctions. 56749 novel splicing junctions.
sampling 85% (121867919) splicing reads. 228010 splicing junctions. 170991 known splicing junctions. 57019 novel splicing junctions.
sampling 90% (129036620) splicing reads. 228255 splicing junctions. 171027 known splicing junctions. 57228 novel splicing junctions.
sampling 95% (136205321) splicing reads. 228440 splicing junctions. 171071 known splicing junctions. 57369 novel splicing junctions.
sampling 100% (143374023) splicing reads. 228533 splicing junctions. 171090 known splicing junctions. 57443 novel splicing junctions.