[W::hts_idx_load3] The index file is older than the data file: 659_bNS-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16903009) splicing reads. 273685 splicing junctions. 192183 known splicing junctions. 81502 novel splicing junctions.
sampling 10% (33806018) splicing reads. 339049 splicing junctions. 205470 known splicing junctions. 133579 novel splicing junctions.
sampling 15% (50709027) splicing reads. 387579 splicing junctions. 212626 known splicing junctions. 174953 novel splicing junctions.
sampling 20% (67612037) splicing reads. 426993 splicing junctions. 217430 known splicing junctions. 209563 novel splicing junctions.
sampling 25% (84515046) splicing reads. 460034 splicing junctions. 220964 known splicing junctions. 239070 novel splicing junctions.
sampling 30% (101418055) splicing reads. 488638 splicing junctions. 223649 known splicing junctions. 264989 novel splicing junctions.
sampling 35% (118321065) splicing reads. 513304 splicing junctions. 225805 known splicing junctions. 287499 novel splicing junctions.
sampling 40% (135224074) splicing reads. 535261 splicing junctions. 227577 known splicing junctions. 307684 novel splicing junctions.
sampling 45% (152127083) splicing reads. 554888 splicing junctions. 229073 known splicing junctions. 325815 novel splicing junctions.
sampling 50% (169030093) splicing reads. 571995 splicing junctions. 230296 known splicing junctions. 341699 novel splicing junctions.
sampling 55% (185933102) splicing reads. 587689 splicing junctions. 231425 known splicing junctions. 356264 novel splicing junctions.
sampling 60% (202836111) splicing reads. 601625 splicing junctions. 232374 known splicing junctions. 369251 novel splicing junctions.
sampling 65% (219739120) splicing reads. 613916 splicing junctions. 233171 known splicing junctions. 380745 novel splicing junctions.
sampling 70% (236642130) splicing reads. 624953 splicing junctions. 233842 known splicing junctions. 391111 novel splicing junctions.
sampling 75% (253545139) splicing reads. 634657 splicing junctions. 234469 known splicing junctions. 400188 novel splicing junctions.
sampling 80% (270448148) splicing reads. 643148 splicing junctions. 234982 known splicing junctions. 408166 novel splicing junctions.
sampling 85% (287351158) splicing reads. 650427 splicing junctions. 235419 known splicing junctions. 415008 novel splicing junctions.
sampling 90% (304254167) splicing reads. 656786 splicing junctions. 235755 known splicing junctions. 421031 novel splicing junctions.
sampling 95% (321157176) splicing reads. 661975 splicing junctions. 236044 known splicing junctions. 425931 novel splicing junctions.
sampling 100% (338060186) splicing reads. 666248 splicing junctions. 236283 known splicing junctions. 429965 novel splicing junctions.