Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/ead7548c923424c68495c616845975/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/de/5b6244ac0271aea817e79ccb3c11d4/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/5e6db1165b7fd12c35ab76c4569de3/659_bTo-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/de/5b6244ac0271aea817e79ccb3c11d4/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/69/282e3130ec2b5936f2c532e47d0011/659_bTo-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: 659_bTo-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18709618) splicing reads. 260744 splicing junctions. 182242 known splicing junctions. 78502 novel splicing junctions.
sampling 10% (37419237) splicing reads. 323306 splicing junctions. 195649 known splicing junctions. 127657 novel splicing junctions.
sampling 15% (56128855) splicing reads. 369512 splicing junctions. 203216 known splicing junctions. 166296 novel splicing junctions.
sampling 20% (74838474) splicing reads. 406095 splicing junctions. 208185 known splicing junctions. 197910 novel splicing junctions.
sampling 25% (93548093) splicing reads. 436703 splicing junctions. 211798 known splicing junctions. 224905 novel splicing junctions.
sampling 30% (112257711) splicing reads. 463087 splicing junctions. 214707 known splicing junctions. 248380 novel splicing junctions.
sampling 35% (130967330) splicing reads. 485730 splicing junctions. 216873 known splicing junctions. 268857 novel splicing junctions.
sampling 40% (149676949) splicing reads. 505689 splicing junctions. 218721 known splicing junctions. 286968 novel splicing junctions.
sampling 45% (168386567) splicing reads. 523126 splicing junctions. 220171 known splicing junctions. 302955 novel splicing junctions.
sampling 50% (187096186) splicing reads. 538676 splicing junctions. 221521 known splicing junctions. 317155 novel splicing junctions.
sampling 55% (205805805) splicing reads. 552543 splicing junctions. 222599 known splicing junctions. 329944 novel splicing junctions.
sampling 60% (224515423) splicing reads. 564959 splicing junctions. 223511 known splicing junctions. 341448 novel splicing junctions.
sampling 65% (243225042) splicing reads. 575679 splicing junctions. 224322 known splicing junctions. 351357 novel splicing junctions.
sampling 70% (261934661) splicing reads. 585513 splicing junctions. 225080 known splicing junctions. 360433 novel splicing junctions.
sampling 75% (280644279) splicing reads. 594207 splicing junctions. 225713 known splicing junctions. 368494 novel splicing junctions.
sampling 80% (299353898) splicing reads. 601707 splicing junctions. 226238 known splicing junctions. 375469 novel splicing junctions.
sampling 85% (318063517) splicing reads. 608205 splicing junctions. 226696 known splicing junctions. 381509 novel splicing junctions.
sampling 90% (336773135) splicing reads. 613678 splicing junctions. 227079 known splicing junctions. 386599 novel splicing junctions.
sampling 95% (355482754) splicing reads. 618354 splicing junctions. 227393 known splicing junctions. 390961 novel splicing junctions.
sampling 100% (374192373) splicing reads. 622054 splicing junctions. 227639 known splicing junctions. 394415 novel splicing junctions.
null device 
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