[W::hts_idx_load3] The index file is older than the data file: 659_b7Z-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17951027) splicing reads. 278099 splicing junctions. 191789 known splicing junctions. 86310 novel splicing junctions.
sampling 10% (35902054) splicing reads. 345614 splicing junctions. 205159 known splicing junctions. 140455 novel splicing junctions.
sampling 15% (53853082) splicing reads. 395276 splicing junctions. 212597 known splicing junctions. 182679 novel splicing junctions.
sampling 20% (71804109) splicing reads. 435704 splicing junctions. 217279 known splicing junctions. 218425 novel splicing junctions.
sampling 25% (89755136) splicing reads. 469761 splicing junctions. 220805 known splicing junctions. 248956 novel splicing junctions.
sampling 30% (107706164) splicing reads. 498958 splicing junctions. 223492 known splicing junctions. 275466 novel splicing junctions.
sampling 35% (125657191) splicing reads. 524526 splicing junctions. 225665 known splicing junctions. 298861 novel splicing junctions.
sampling 40% (143608218) splicing reads. 547073 splicing junctions. 227470 known splicing junctions. 319603 novel splicing junctions.
sampling 45% (161559246) splicing reads. 567191 splicing junctions. 228989 known splicing junctions. 338202 novel splicing junctions.
sampling 50% (179510273) splicing reads. 585032 splicing junctions. 230250 known splicing junctions. 354782 novel splicing junctions.
sampling 55% (197461300) splicing reads. 601051 splicing junctions. 231298 known splicing junctions. 369753 novel splicing junctions.
sampling 60% (215412328) splicing reads. 615757 splicing junctions. 232246 known splicing junctions. 383511 novel splicing junctions.
sampling 65% (233363355) splicing reads. 628624 splicing junctions. 233081 known splicing junctions. 395543 novel splicing junctions.
sampling 70% (251314382) splicing reads. 640362 splicing junctions. 233783 known splicing junctions. 406579 novel splicing junctions.
sampling 75% (269265410) splicing reads. 650751 splicing junctions. 234377 known splicing junctions. 416374 novel splicing junctions.
sampling 80% (287216437) splicing reads. 659932 splicing junctions. 234872 known splicing junctions. 425060 novel splicing junctions.
sampling 85% (305167464) splicing reads. 668181 splicing junctions. 235334 known splicing junctions. 432847 novel splicing junctions.
sampling 90% (323118492) splicing reads. 675343 splicing junctions. 235751 known splicing junctions. 439592 novel splicing junctions.
sampling 95% (341069519) splicing reads. 681445 splicing junctions. 236087 known splicing junctions. 445358 novel splicing junctions.
sampling 100% (359020547) splicing reads. 686463 splicing junctions. 236356 known splicing junctions. 450107 novel splicing junctions.