[W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15820921) splicing reads. 262683 splicing junctions. 186113 known splicing junctions. 76570 novel splicing junctions.
sampling 10% (31641843) splicing reads. 325470 splicing junctions. 199449 known splicing junctions. 126021 novel splicing junctions.
sampling 15% (47462765) splicing reads. 371753 splicing junctions. 206942 known splicing junctions. 164811 novel splicing junctions.
sampling 20% (63283687) splicing reads. 409158 splicing junctions. 211941 known splicing junctions. 197217 novel splicing junctions.
sampling 25% (79104609) splicing reads. 440693 splicing junctions. 215579 known splicing junctions. 225114 novel splicing junctions.
sampling 30% (94925531) splicing reads. 467728 splicing junctions. 218273 known splicing junctions. 249455 novel splicing junctions.
sampling 35% (110746453) splicing reads. 490895 splicing junctions. 220493 known splicing junctions. 270402 novel splicing junctions.
sampling 40% (126567375) splicing reads. 511707 splicing junctions. 222312 known splicing junctions. 289395 novel splicing junctions.
sampling 45% (142388297) splicing reads. 529864 splicing junctions. 223860 known splicing junctions. 306004 novel splicing junctions.
sampling 50% (158209219) splicing reads. 545824 splicing junctions. 225107 known splicing junctions. 320717 novel splicing junctions.
sampling 55% (174030141) splicing reads. 560159 splicing junctions. 226264 known splicing junctions. 333895 novel splicing junctions.
sampling 60% (189851063) splicing reads. 572941 splicing junctions. 227258 known splicing junctions. 345683 novel splicing junctions.
sampling 65% (205671985) splicing reads. 584139 splicing junctions. 228019 known splicing junctions. 356120 novel splicing junctions.
sampling 70% (221492907) splicing reads. 594101 splicing junctions. 228722 known splicing junctions. 365379 novel splicing junctions.
sampling 75% (237313829) splicing reads. 602817 splicing junctions. 229331 known splicing junctions. 373486 novel splicing junctions.
sampling 80% (253134751) splicing reads. 610417 splicing junctions. 229851 known splicing junctions. 380566 novel splicing junctions.
sampling 85% (268955673) splicing reads. 616893 splicing junctions. 230304 known splicing junctions. 386589 novel splicing junctions.
sampling 90% (284776595) splicing reads. 622450 splicing junctions. 230676 known splicing junctions. 391774 novel splicing junctions.
sampling 95% (300597517) splicing reads. 627007 splicing junctions. 231011 known splicing junctions. 395996 novel splicing junctions.
sampling 100% (316418439) splicing reads. 630461 splicing junctions. 231257 known splicing junctions. 399204 novel splicing junctions.