Done. -parsing GTF file: /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.YDu93R4Uaz/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1_1.fastp.fastq.gz /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.YDu93R4Uaz/fi_workdir/659_bhK-T1-TRNA-1_B23MHV2LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.YDu93R4Uaz/fi_workdir/659_bhK-T1-TRNA-1_B23MHV2LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam 659_bhK-T1-TRNA-1_B23MHV2LT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index 659_bhK-T1-TRNA-1_B23MHV2LT4_1.star.sortedByCoord.out.bam 23:33:47 : INFO : Done. -parsing GTF file: /tmp/nxf.YDu93R4Uaz/fi_workdir/659_bhK-T1-TRNA-1_B23MHV2LT4_1.gtf -parsing /tmp/nxf.YDu93R4Uaz/fi_workdir/659_bhK-T1-TRNA-1_B23MHV2LT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] [500000] [510000] [520000] [530000] [540000] [550000] -done parsing /tmp/nxf.YDu93R4Uaz/fi_workdir/659_bhK-T1-TRNA-1_B23MHV2LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'per_id < 96' => 2711, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 22, 'low complexity anchor region' => 1, 'small anchor length' => 47, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 1, 'excessive soft clipping' => 3577, 'seq-similar region overlap' => 39, 'num genes matched < 2' => 24, ' ** passed ** ' => 5415 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.YDu93R4Uaz/fi_workdir/659_bhK-T1-TRNA-1_B23MHV2LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] [125000] [126000] [127000] [128000] [129000] [130000] [131000] [132000] [133000] [134000] [135000] [136000] [137000] [138000] [139000] [140000] [141000] [142000] [143000] [144000] [145000] [146000] [147000] [148000] [149000] [150000] [151000] [152000] [153000] [154000] [155000] [156000] [157000] [158000] [159000] [160000] [161000] [162000] [163000] [164000] [165000] [166000] [167000] [168000] [169000] [170000] [171000] [172000] [173000] [174000] [175000] [176000] -fusion SPANNING read extraction for scaff: CCDC6--RET [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] -fusion SPANNING read extraction for scaff: DNAJC1--C10orf143 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: ECHS1--NEBL [1000] [2000] [3000] -fusion SPANNING read extraction for scaff: KLRC4--KLRK1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] -fusion SPANNING read extraction for scaff: NEBL--CDK1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGA8 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGA10 [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGC4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGB6 [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGC5 [1000] [2000] [3000] [4000] [5000] [6000] [7000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGA11 [1000] [2000] [3000] [4000] [5000] [6000] [7000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGB7 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGC3 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGA9 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGA12 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: PMS2P5--CCDC146 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] -fusion SPANNING read extraction for scaff: PRSS27--TPSB2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] -fusion SPANNING read extraction for scaff: STX16--NPEPL1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] -fusion SPANNING read extraction for scaff: TLK2--FAM157A [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: TRIM59--IFT80 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 2220, 'seq similar region alignment' => 7629 }; EM: Starting log likelihood: -4004.318034 EM: Round [1] log likelihood: -3998.058170 EM: Round [2] log likelihood: -3997.701292 EM: Round [3] log likelihood: -3997.413502 EM: Round [4] log likelihood: -3997.173934 EM: Round [5] log likelihood: -3996.973673 EM: Round [6] log likelihood: -3996.805633 EM: Round [7] log likelihood: -3996.664124 EM: Round [8] log likelihood: -3996.544553 EM: Round [9] log likelihood: -3996.443188 EM: Round [10] log likelihood: -3996.356986 EM: Round [11] log likelihood: -3996.283452 EM: Round [12] log likelihood: -3996.220538 EM: Round [13] log likelihood: -3996.166553 EM: Round [14] log likelihood: -3996.120096 EM: Round [15] log likelihood: -3996.080007 EM: Round [16] log likelihood: -3996.045320 EM: Round [17] log likelihood: -3996.015226 EM: Round [18] log likelihood: -3995.989051 EM: Round [19] log likelihood: -3995.966228 EM: Round [20] log likelihood: -3995.946280 EM: Round [21] log likelihood: -3995.928806 EM: Round [22] log likelihood: -3995.913464 EM: Round [23] log likelihood: -3995.899966 EM: Round [24] log likelihood: -3995.888068 EM: Round [25] log likelihood: -3995.877558 EM: Round [26] log likelihood: -3995.868260 EM: Round [27] log likelihood: -3995.860019 EM: Round [28] log likelihood: -3995.852703 EM: Round [29] log likelihood: -3995.846199 EM: Round [30] log likelihood: -3995.840410 EM: Round [31] log likelihood: -3995.835249 EM: Round [32] log likelihood: -3995.830642 EM: Round [33] log likelihood: -3995.826527 EM: Round [34] log likelihood: -3995.822846 EM: Round [35] log likelihood: -3995.819550 EM: Round [36] log likelihood: -3995.816597 EM: Round [37] log likelihood: -3995.813949 EM: Round [38] log likelihood: -3995.811572 EM: Round [39] log likelihood: -3995.809438 EM: Round [40] log likelihood: -3995.807519 EM: Round [41] log likelihood: -3995.805794 EM: Round [42] log likelihood: -3995.804242 EM: Round [43] log likelihood: -3995.802844 EM: Round [44] log likelihood: -3995.801586 EM: Round [45] log likelihood: -3995.800452 EM: Round [46] log likelihood: -3995.799429 EM: Round [47] log likelihood: -3995.798507 EM: Round [48] log likelihood: -3995.797676 EM: Round [49] log likelihood: -3995.796925 EM: Round [50] log likelihood: -3995.796248 EM: Round [51] log likelihood: -3995.795636 EM: Round [52] log likelihood: -3995.795083 EM: Round [53] log likelihood: -3995.794584 EM: Round [54] log likelihood: -3995.794133 EM: Round [55] log likelihood: -3995.793725 EM: Round [56] log likelihood: -3995.793356 EM: Round [57] log likelihood: -3995.793022 EM: Round [58] log likelihood: -3995.792720 EM: Round [59] log likelihood: -3995.792446 EM: Round [60] log likelihood: -3995.792199 EM: Round [61] log likelihood: -3995.791975 EM: Round [62] log likelihood: -3995.791771 EM: Round [63] log likelihood: -3995.791587 EM: Round [64] log likelihood: -3995.791420 EM: Round [65] log likelihood: -3995.791269 EM: Round [66] log likelihood: -3995.791132 EM: Round [67] log likelihood: -3995.791007 EM: Round [68] log likelihood: -3995.790894 EM: Round [69] log likelihood: -3995.790791 EM: Round [70] log likelihood: -3995.790698 EM: Stopping iterations at round 70 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.consolidated.bam into /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.consolidated.bam.read_coords > /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.consolidated.bam.frag_coords > /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.YDu93R4Uaz/fi_workdir/659_bhK-T1-TRNA-1_B23MHV2LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.YDu93R4Uaz/fi_workdir/659_bhK-T1-TRNA-1_B23MHV2LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.YDu93R4Uaz/fi_workdir/659_bhK-T1-TRNA-1_B23MHV2LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -3851.186795 EM: Round [1] log likelihood: -3850.284131 EM: Round [2] log likelihood: -3850.279926 EM: Round [3] log likelihood: -3850.279911 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1_1.fastp.fastq.gz: 295280014 Warning - not locating file: /tmp/nxf.YDu93R4Uaz/659_bhK-T1-TRNA-1_B23MHV2LT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000123965.13] WARNING, no entry stored in dbm for [ENSG00000123965.13] WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] ls: cannot access 'IGV_inputs': No such file or directory