Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b9/f1a140e26752d178425115b4293a36/659_bR4-T1-TRNA-1_B23MHV2LT4_1_1.fastp.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/94/1e7a073df68f2c1a6261e2fd036c73/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/94/1e7a073df68f2c1a6261e2fd036c73/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b9/f1a140e26752d178425115b4293a36/659_bR4-T1-TRNA-1_B23MHV2LT4_1_2.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) STAR --genomeDir ref_genome.fa.star.idx --readFilesIn input1/659_bR4-T1-TRNA-1_B23MHV2LT4_1_1.fastp.fastq.gz input2/659_bR4-T1-TRNA-1_B23MHV2LT4_1_2.fastp.fastq.gz --runThreadN 24 --outFileNamePrefix 659_bR4-T1-TRNA-1_B23MHV2LT4_1. --sjdbGTFfile ref_annot.gtf --outSAMattrRGline ID:659_bR4-T1-TRNA-1_B23MHV2LT4_1 SM:659_bR4-T1-TRNA-1_B23MHV2LT4_1 --twopassMode None --chimSegmentMin 12 --chimJunctionOverhangMin 5 --chimMultimapNmax 50 --chimScoreJunctionNonGTAG -4 --chimScoreSeparation 1 --alignIntronMax 140000 --outReadsUnmapped None --readFilesCommand zcat --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outSAMunmapped Within --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --chimMultimapScoreRange 3 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLmin 30 --alignSplicedMateMapLminOverLmate 0.5 --quantMode GeneCounts --runRNGseed 1 --chimOutType Junctions WithinBAM --limitBAMsortRAM 38654705664 STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source Jun 04 20:27:06 ..... started STAR run Jun 04 20:27:06 ..... loading genome Jun 04 20:27:23 ..... processing annotations GTF Jun 04 20:27:31 ..... inserting junctions into the genome indices Jun 04 20:28:46 ..... started mapping Jun 04 21:02:02 ..... finished mapping Jun 04 21:02:04 ..... started sorting BAM Jun 04 21:06:10 ..... finished successfully