.command.log - Workdir - NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STARFUSION (NTC_0001_0001_B23MHV2LT4_1)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/48/955227b6ce5305702d45b3a765523f/.command.log
Size: 8.5 KB
Task: NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STARFUSION (NTC_0001_0001_B23MHV2LT4_1)
Attempt
Content

  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a3/785cad82815fb1185eb0d20d6c5f9e/NTC_0001_0001_B23MHV2LT4_1.Chimeric.out.junction
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/70/9ed237a5c08ea74ef5231c58215a58/NTC_0001_0001_B23MHV2LT4_1_2.fastp.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/48/955227b6ce5305702d45b3a765523f/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/70/9ed237a5c08ea74ef5231c58215a58/NTC_0001_0001_B23MHV2LT4_1_1.fastp.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/48/955227b6ce5305702d45b3a765523f/.command.run
==> STAGING COMPLETE (6 inputs)

-ctat genome lib [/tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir] validates.
CMD: mkdir -p /tmp/nxf.Frh2RLgMFx/./_starF_checkpoints
CMD: mkdir -p /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary
-sample contains 23560 rnaseq fragments
* Running CMD: /usr/local/src/STAR-Fusion/util/STAR-Fusion.map_chimeric_reads_to_genes  --genome_lib_dir /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir  -J /tmp/nxf.Frh2RLgMFx/NTC_0001_0001_B23MHV2LT4_1.Chimeric.out.junction  -O /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.junction_breakpts_to_genes.txt --CPU 4 
-parsing fusion evidence: /tmp/nxf.Frh2RLgMFx/NTC_0001_0001_B23MHV2LT4_1.Chimeric.out.junction
-building interval tree based on /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-done building interval tree (0.05 min).
-mapping reads to genes
-building interval tree based on /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-done building interval tree (0.05 min).
-mapping reads to genes
-building interval tree based on /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-done building interval tree (0.05 min).
-mapping reads to genes
-building interval tree based on /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-done building interval tree (0.05 min).
-mapping reads to genes
* Running CMD: /usr/local/src/STAR-Fusion/util/STAR-Fusion.handle_multimapping_reads  -J /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.junction_breakpts_to_genes.txt  --genome_lib_dir /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir  --filt_file /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.junction_breakpts_to_genes.txt.fail  -M 50  > /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.junction_breakpts_to_genes.txt.pass 
-parsing /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.junction_breakpts_to_genes.txt, organizing read mapping info.

done.

* Running CMD: /usr/local/src/STAR-Fusion/util/STAR-Fusion.predict  -J /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.junction_breakpts_to_genes.txt.pass  -O /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion 
-parsing fusion evidence: /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.junction_breakpts_to_genes.txt.pass
-outputting fusion candidates to file: /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary
* Running CMD: /usr/local/src/STAR-Fusion/util/STAR-Fusion.filter  --fusion_preds /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary  -J /tmp/nxf.Frh2RLgMFx/NTC_0001_0001_B23MHV2LT4_1.Chimeric.out.junction  --tmpdir /tmp  --min_junction_reads 0  --min_sum_frags 1  --require_LDAS 0  --max_promiscuity 10  --min_pct_dom_promiscuity 20  --min_novel_junction_support 1  --min_alt_pct_junction 10  --aggregate_novel_junction_dist 5  --min_spanning_frags_only 1  --genome_lib_dir /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir  --out_prefix /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion
CMD: mkdir -p /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.filter.intermediates_dir
EM: Starting log likelihood: -27.513319
EM: Round [1] log likelihood: -27.513319
EM: Stopping iterations at round 1 due to insufficient improvement in likelihood.
CMD: /usr/local/src/STAR-Fusion/util/../FusionFilter/blast_and_promiscuity_filter.pl --out_prefix /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.filter.intermediates_dir/star-fusion --max_promiscuity 10 --min_pct_dom_promiscuity 20  --fusion_preds /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.filter.intermediates_dir/star-fusion.pre_blast_filter --genome_lib_dir /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir 
CMD: /usr/local/src/STAR-Fusion/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.filter.intermediates_dir/star-fusion.pre_blast_filter --genome_lib_dir /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.filter.intermediates_dir/star-fusion.pre_blast_filter.post_blast_filter  --genome_lib_dir /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir  --max_promiscuity 10  --min_pct_dom_promiscuity 20 
CMD: cp /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.filter.intermediates_dir/star-fusion.pre_blast_filter.post_blast_filter.post_promisc_filter /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.filter.intermediates_dir/star-fusion.post_blast_and_promiscuity_filter
CMD: /usr/local/src/STAR-Fusion/util/column_exclusions.pl /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.filter.intermediates_dir/star-fusion.post_blast_and_promiscuity_filter JunctionReads,SpanningFrags > /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.filter.intermediates_dir/star-fusion.post_blast_and_promiscuity_filter.abridged
CMD: ln -sf /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.filter.intermediates_dir/star-fusion.post_blast_and_promiscuity_filter /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.filtered
* Running CMD: /usr/local/src/STAR-Fusion/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.filtered 23560 > /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.filtered.FFPM
* Running CMD: /usr/local/src/STAR-Fusion/util/append_breakpoint_junction_info.pl /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.filtered.FFPM /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir > /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo
* Running CMD: /usr/local/src/STAR-Fusion/FusionAnnotator/FusionAnnotator --genome_lib_dir /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir --annotate /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo > /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo.wAnnot
* Running CMD: /usr/local/src/STAR-Fusion/FusionFilter/util/filter_by_annotation_rules.pl --fusions /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo.wAnnot --genome_lib_dir /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir
-done, see /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo.wAnnot.annot_filter.pass
* Running CMD: /usr/local/src/STAR-Fusion/util/filter_likely_RT_artifacts.pl --fusions /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo.wAnnot.annot_filter.pass --genome_lib_dir /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir
-filter_likely_RT_artifacts: (pass: 1, filtered: 18)
* Running CMD: /usr/local/src/STAR-Fusion/util/filter_by_min_FFPM.pl /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo.wAnnot.annot_filter.pass.RTartifact.pass 0.00001
* Running CMD: cp /tmp/nxf.Frh2RLgMFx/./star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo.wAnnot.annot_filter.pass.RTartifact.pass.minFFPM.0.00001.pass star-fusion.fusion_predictions.tsv
* Running CMD: /usr/local/src/STAR-Fusion/util/column_exclusions.pl star-fusion.fusion_predictions.tsv JunctionReads,SpanningFrags > star-fusion.fusion_predictions.abridged.tsv
* Running CMD: /usr/local/src/STAR-Fusion/FusionAnnotator/util/fusion_to_coding_region_effect.pl  --fusions star-fusion.fusion_predictions.abridged.tsv --genome_lib_dir /tmp/nxf.Frh2RLgMFx/ctat_genome_lib_build_dir > star-fusion.fusion_predictions.abridged.coding_effect.tsv


	* STAR-Fusion complete.  See output: /tmp/nxf.Frh2RLgMFx/./star-fusion.fusion_predictions.tsv (or .abridged.tsv version)