Using GATK jar /usr/local/share/gatk4-4.4.0.0-0/gatk-package-4.4.0.0-local.jar Running: java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -Xmx60620M -XX:-UsePerfData -XX:ParallelGCThreads=2 -jar /usr/local/share/gatk4-4.4.0.0-0/gatk-package-4.4.0.0-local.jar MarkDuplicates --INPUT 659_8h-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam --OUTPUT 659_8h-T1-TRNA-1_B23MHV2LT4_1.md.bam --METRICS_FILE 659_8h-T1-TRNA-1_B23MHV2LT4_1.md.bam.metrics --TMP_DIR . --CREATE_INDEX true --REFERENCE_SEQUENCE ref_genome.fa -REMOVE_DUPLICATES false -VALIDATION_STRINGENCY LENIENT 20:20:19.360 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/usr/local/share/gatk4-4.4.0.0-0/gatk-package-4.4.0.0-local.jar!/com/intel/gkl/native/libgkl_compression.so [Thu Jun 04 20:20:19 GMT 2026] MarkDuplicates --INPUT 659_8h-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam --OUTPUT 659_8h-T1-TRNA-1_B23MHV2LT4_1.md.bam --METRICS_FILE 659_8h-T1-TRNA-1_B23MHV2LT4_1.md.bam.metrics --REMOVE_DUPLICATES false --TMP_DIR . --VALIDATION_STRINGENCY LENIENT --CREATE_INDEX true --REFERENCE_SEQUENCE ref_genome.fa --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --COMPRESSION_LEVEL 2 --MAX_RECORDS_IN_RAM 500000 --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false [Thu Jun 04 20:20:19 GMT 2026] Executing as ?@nf-6bab10ac466750f1c9c12a7d99fe4e0f-cde77-2hrb9 on Linux 6.12.88 amd64; OpenJDK 64-Bit Server VM 17.0.3-internal+0-adhoc..src; Deflater: Intel; Inflater: Intel; Provider GCS is available; Picard version: Version:4.4.0.0 INFO 2026-06-04 20:20:19 MarkDuplicates Start of doWork freeMemory: 105828768; totalMemory: 134217728; maxMemory: 63585648640 INFO 2026-06-04 20:20:19 MarkDuplicates Reading input file and constructing read end information. INFO 2026-06-04 20:20:19 MarkDuplicates Will retain up to 230382784 data points before spilling to disk. INFO 2026-06-04 20:20:21 MarkDuplicates Read 483846 records. 0 pairs never matched. INFO 2026-06-04 20:20:21 MarkDuplicates After buildSortedReadEndLists freeMemory: 2531978328; totalMemory: 6274678784; maxMemory: 63585648640 INFO 2026-06-04 20:20:21 MarkDuplicates Will retain up to 1987051520 duplicate indices before spilling to disk. INFO 2026-06-04 20:20:27 MarkDuplicates Traversing read pair information and detecting duplicates. INFO 2026-06-04 20:20:27 OpticalDuplicateFinder Large duplicate set. size = 1599 INFO 2026-06-04 20:20:27 OpticalDuplicateFinder compared 1,000 ReadEnds to others. Elapsed time: 00:00:00s. Time for last 1,000: 0s. Last read position: 0:27,479 INFO 2026-06-04 20:20:27 OpticalDuplicateFinder Large duplicate set. size = 1468 INFO 2026-06-04 20:20:27 OpticalDuplicateFinder compared 1,000 ReadEnds to others. Elapsed time: 00:00:00s. Time for last 1,000: 0s. Last read position: 0:24,777 INFO 2026-06-04 20:20:27 OpticalDuplicateFinder Large duplicate set. size = 1305 INFO 2026-06-04 20:20:27 OpticalDuplicateFinder compared 1,000 ReadEnds to others. Elapsed time: 00:00:00s. Time for last 1,000: 0s. Last read position: 0:30,213 INFO 2026-06-04 20:20:27 MarkDuplicates Traversing fragment information and detecting duplicates. INFO 2026-06-04 20:20:27 MarkDuplicates Sorting list of duplicate records. INFO 2026-06-04 20:20:27 MarkDuplicates After generateDuplicateIndexes freeMemory: 16899821008; totalMemory: 32849788928; maxMemory: 63585648640 INFO 2026-06-04 20:20:27 MarkDuplicates Marking 46899 records as duplicates. INFO 2026-06-04 20:20:27 MarkDuplicates Found 145 optical duplicate clusters. INFO 2026-06-04 20:20:27 MarkDuplicates Reads are assumed to be ordered by: coordinate INFO 2026-06-04 20:20:28 MarkDuplicates Writing complete. Closing input iterator. INFO 2026-06-04 20:20:28 MarkDuplicates Duplicate Index cleanup. INFO 2026-06-04 20:20:28 MarkDuplicates Getting Memory Stats. INFO 2026-06-04 20:20:28 MarkDuplicates Before output close freeMemory: 202610424; totalMemory: 234881024; maxMemory: 63585648640 INFO 2026-06-04 20:20:28 MarkDuplicates Closed outputs. Getting more Memory Stats. INFO 2026-06-04 20:20:28 MarkDuplicates After output close freeMemory: 105286672; totalMemory: 134217728; maxMemory: 63585648640 [Thu Jun 04 20:20:28 GMT 2026] picard.sam.markduplicates.MarkDuplicates done. Elapsed time: 0.15 minutes. Runtime.totalMemory()=134217728 ls: *cram: No such file or directory ls: *.crai: No such file or directory