[W::hts_idx_load3] The index file is older than the data file: 659_bIB-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18803150) splicing reads. 270029 splicing junctions. 186216 known splicing junctions. 83813 novel splicing junctions.
sampling 10% (37606301) splicing reads. 337355 splicing junctions. 199638 known splicing junctions. 137717 novel splicing junctions.
sampling 15% (56409452) splicing reads. 387613 splicing junctions. 207022 known splicing junctions. 180591 novel splicing junctions.
sampling 20% (75212603) splicing reads. 428522 splicing junctions. 211911 known splicing junctions. 216611 novel splicing junctions.
sampling 25% (94015753) splicing reads. 462978 splicing junctions. 215590 known splicing junctions. 247388 novel splicing junctions.
sampling 30% (112818904) splicing reads. 493067 splicing junctions. 218375 known splicing junctions. 274692 novel splicing junctions.
sampling 35% (131622055) splicing reads. 519544 splicing junctions. 220618 known splicing junctions. 298926 novel splicing junctions.
sampling 40% (150425206) splicing reads. 542857 splicing junctions. 222466 known splicing junctions. 320391 novel splicing junctions.
sampling 45% (169228356) splicing reads. 563908 splicing junctions. 224031 known splicing junctions. 339877 novel splicing junctions.
sampling 50% (188031507) splicing reads. 582805 splicing junctions. 225344 known splicing junctions. 357461 novel splicing junctions.
sampling 55% (206834658) splicing reads. 599827 splicing junctions. 226502 known splicing junctions. 373325 novel splicing junctions.
sampling 60% (225637809) splicing reads. 614984 splicing junctions. 227449 known splicing junctions. 387535 novel splicing junctions.
sampling 65% (244440959) splicing reads. 628546 splicing junctions. 228287 known splicing junctions. 400259 novel splicing junctions.
sampling 70% (263244110) splicing reads. 640889 splicing junctions. 229047 known splicing junctions. 411842 novel splicing junctions.
sampling 75% (282047261) splicing reads. 651606 splicing junctions. 229707 known splicing junctions. 421899 novel splicing junctions.
sampling 80% (300850412) splicing reads. 661252 splicing junctions. 230257 known splicing junctions. 430995 novel splicing junctions.
sampling 85% (319653562) splicing reads. 669799 splicing junctions. 230761 known splicing junctions. 439038 novel splicing junctions.
sampling 90% (338456713) splicing reads. 677314 splicing junctions. 231222 known splicing junctions. 446092 novel splicing junctions.
sampling 95% (357259864) splicing reads. 683733 splicing junctions. 231586 known splicing junctions. 452147 novel splicing junctions.
sampling 100% (376063015) splicing reads. 689215 splicing junctions. 231904 known splicing junctions. 457311 novel splicing junctions.