+ mkdir 659_bMa-T1-TRNA-1_B23MHV2LT4_1 + all_qc -bam 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder 659_bMa-T1-TRNA-1_B23MHV2LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai 0it [00:00, ?it/s] 164509it [00:00, 1644969.99it/s][W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai 341159it [00:00, 1716418.53it/s] 522613it [00:00, 1761195.52it/s] 705551it [00:00, 1788092.08it/s] 886659it [00:00, 1796289.45it/s] 1067079it [00:00, 1798957.62it/s] 1248532it [00:00, 1804032.07it/s] 1428936it [00:00, 1794921.23it/s] 1608862it [00:00, 1796254.70it/s] 1791060it [00:01, 1804157.99it/s] 1973069it [00:01, 1808947.21it/s] 2156321it [00:01, 1816093.05it/s] 2337935it [00:01, 1813668.15it/s] 2521287it [00:01, 1819506.57it/s] 2703648it [00:01, 1820720.38it/s][W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai 2885723it [00:01, 1816163.89it/s] 3067344it [00:01, 1792212.93it/s] 3247479it [00:01, 1794912.64it/s] 3429175it [00:01, 1801467.67it/s] 3609363it [00:02, 1786687.42it/s] 3790591it [00:02, 1794278.81it/s] 3971476it [00:02, 1798606.42it/s][W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai 4154386it [00:02, 1807696.37it/s] 4336616it [00:02, 1812048.80it/s] 4517841it [00:02, 1810129.11it/s] 4700830it [00:02, 1816028.72it/s] 4882445it [00:02, 1809922.47it/s] 5063666it [00:02, 1810591.36it/s] 5244734it [00:02, 1805145.83it/s] 5426723it [00:03, 1809538.44it/s] 5609185it [00:03, 1813905.02it/s] 5791387it [00:03, 1816321.04it/s] 5974267it [00:03, 1819928.84it/s] 6156264it [00:03, 1812266.65it/s] 6337501it [00:03, 1804229.90it/s] 6517936it [00:03, 1802969.87it/s] 6698241it [00:03, 1792946.96it/s] 6877550it [00:03, 1790803.75it/s] 7056640it [00:03, 1788344.22it/s] 7235481it [00:04, 1778602.87it/s] 7414664it [00:04, 1782514.52it/s] 7596055it [00:04, 1791860.80it/s][W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai 7777019it [00:04, 1797111.67it/s] 7961078it [00:04, 1810104.10it/s][W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai 8113870it [00:04, 1801320.16it/s] [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai + merge_qc -folders 659_bMa-T1-TRNA-1_B23MHV2LT4_1/659_bMa-T1-TRNA-1_B23MHV2LT4_1.md -output 659_bMa-T1-TRNA-1_B23MHV2LT4_1/659_bMa-T1-TRNA-1_B23MHV2LT4_1_full_basic_stats.tsv + head -n1 659_bMa-T1-TRNA-1_B23MHV2LT4_1/659_bMa-T1-TRNA-1_B23MHV2LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed 659_bMa-T1-TRNA-1_B23MHV2LT4_1/659_bMa-T1-TRNA-1_B23MHV2LT4_1_full_basic_stats.tsv