[W::hts_idx_load3] The index file is older than the data file: 659_bQZ-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17211035) splicing reads. 282479 splicing junctions. 191140 known splicing junctions. 91339 novel splicing junctions.
sampling 10% (34422070) splicing reads. 349495 splicing junctions. 204032 known splicing junctions. 145463 novel splicing junctions.
sampling 15% (51633105) splicing reads. 397083 splicing junctions. 211128 known splicing junctions. 185955 novel splicing junctions.
sampling 20% (68844141) splicing reads. 433998 splicing junctions. 215636 known splicing junctions. 218362 novel splicing junctions.
sampling 25% (86055176) splicing reads. 464794 splicing junctions. 219025 known splicing junctions. 245769 novel splicing junctions.
sampling 30% (103266211) splicing reads. 490198 splicing junctions. 221552 known splicing junctions. 268646 novel splicing junctions.
sampling 35% (120477247) splicing reads. 511745 splicing junctions. 223453 known splicing junctions. 288292 novel splicing junctions.
sampling 40% (137688282) splicing reads. 530244 splicing junctions. 225056 known splicing junctions. 305188 novel splicing junctions.
sampling 45% (154899317) splicing reads. 546326 splicing junctions. 226345 known splicing junctions. 319981 novel splicing junctions.
sampling 50% (172110353) splicing reads. 560396 splicing junctions. 227467 known splicing junctions. 332929 novel splicing junctions.
sampling 55% (189321388) splicing reads. 572603 splicing junctions. 228353 known splicing junctions. 344250 novel splicing junctions.
sampling 60% (206532423) splicing reads. 583276 splicing junctions. 229134 known splicing junctions. 354142 novel splicing junctions.
sampling 65% (223743458) splicing reads. 592719 splicing junctions. 229825 known splicing junctions. 362894 novel splicing junctions.
sampling 70% (240954494) splicing reads. 600927 splicing junctions. 230374 known splicing junctions. 370553 novel splicing junctions.
sampling 75% (258165529) splicing reads. 607985 splicing junctions. 230857 known splicing junctions. 377128 novel splicing junctions.
sampling 80% (275376564) splicing reads. 614065 splicing junctions. 231279 known splicing junctions. 382786 novel splicing junctions.
sampling 85% (292587600) splicing reads. 619265 splicing junctions. 231637 known splicing junctions. 387628 novel splicing junctions.
sampling 90% (309798635) splicing reads. 623730 splicing junctions. 231930 known splicing junctions. 391800 novel splicing junctions.
sampling 95% (327009670) splicing reads. 627333 splicing junctions. 232183 known splicing junctions. 395150 novel splicing junctions.
sampling 100% (344220706) splicing reads. 630148 splicing junctions. 232395 known splicing junctions. 397753 novel splicing junctions.