[2026-06-04T21:17:45] Launching Arriba 2.4.0
[2026-06-04T21:17:45] Loading assembly from 'ref_genome.fa' 
[2026-06-04T21:17:53] Loading annotation from 'ref_annot.gtf' 
[2026-06-04T21:17:56] Reading chimeric alignments from '659_dHy-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam' (total=10637541)
[2026-06-04T21:24:41] Marking multi-mapping alignments (marked=6776307)
[2026-06-04T21:24:45] Detecting strandedness (reverse)
[2026-06-04T21:24:45] Assigning strands to alignments 
[2026-06-04T21:24:47] Annotating alignments 
[2026-06-04T21:25:21] Filtering duplicates (remaining=6784429)
[2026-06-04T21:25:28] Filtering mates which do not map to interesting contigs (1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y AC_* NC_*) (remaining=6377083)
[2026-06-04T21:25:30] Filtering mates which only map to viral contigs (AC_* NC_*) (remaining=6377083)
[2026-06-04T21:25:32] Filtering viral contigs with expression lower than the top 5 (remaining=6377083)
[2026-06-04T21:25:37] Filtering viral contigs with less than 5% coverage (remaining=6377083)
[2026-06-04T21:25:40] Estimating fragment length (mate gap mean=-86.9471, mate gap stddev=29.2116, read length mean=134.341)
[2026-06-04T21:25:40] Filtering read-through fragments with a distance <=10000bp (remaining=6024892)
[2026-06-04T21:25:42] Filtering inconsistently clipped mates (remaining=5907890)
[2026-06-04T21:25:44] Filtering breakpoints adjacent to homopolymers >=6nt (remaining=5684128)
[2026-06-04T21:25:49] Filtering fragments with small insert size (remaining=5683379)
[2026-06-04T21:25:51] Filtering alignments with long gaps (remaining=5683379)
[2026-06-04T21:25:53] Filtering fragments with both mates in the same gene (remaining=5682272)
[2026-06-04T21:25:55] Filtering fusions arising from hairpin structures (remaining=5240361)
[2026-06-04T21:25:58] Filtering reads with a mismatch p-value <=0.01 (remaining=2871452)
[2026-06-04T21:26:12] Filtering reads with low entropy (k-mer content >=60%) (remaining=1275710)
[2026-06-04T21:26:23] Finding fusions and counting supporting reads (total=818863)
[2026-06-04T21:26:38] Merging adjacent fusion breakpoints (remaining=808529)
[2026-06-04T21:26:40] Filtering multi-mapping fusions by alignment score and read support (remaining=528386)
[2026-06-04T21:27:09] Estimating expected number of fusions by random chance (e-value) 
[2026-06-04T21:27:16] Filtering fusions with both breakpoints in adjacent non-coding/intergenic regions (remaining=525156)
[2026-06-04T21:27:16] Filtering intragenic fusions with both breakpoints in exonic regions (remaining=297084)
[2026-06-04T21:27:18] Filtering fusions with <2 supporting reads (remaining=44283)
[2026-06-04T21:27:18] Filtering fusions with an e-value >=0.3 (remaining=11915)
[2026-06-04T21:27:19] Searching for internal tandem duplications <=100bp with >=10 supporting reads and >=7% allele fraction (remaining=11984)
[2026-06-04T21:27:23] Filtering fusions with both breakpoints in intronic/intergenic regions (remaining=11843)
[2026-06-04T21:27:23] Searching for known fusions in 'known_fusions_hg38_GRCh38_v2.4.0.tsv.gz' (remaining=11843)
[2026-06-04T21:27:25] Filtering in vitro-generated fusions between genes with an expression above the 99.8% quantile (remaining=9512)
[2026-06-04T21:27:30] Searching for fusions with spliced split reads (remaining=9560)
[2026-06-04T21:27:35] Selecting best breakpoints from genes with multiple breakpoints (remaining=5532)
[2026-06-04T21:27:36] Filtering read-through fusions with breakpoints near the gene boundary (remaining=5463)
[2026-06-04T21:27:37] Searching for fusions with >=4 spliced events (remaining=6709)
[2026-06-04T21:27:38] Filtering blacklisted fusions in 'blacklist_hg38_GRCh38_v2.4.0.tsv.gz' (remaining=2361)
[2026-06-04T21:27:53] Filtering fusions with anchors <=23nt (remaining=1897)
[2026-06-04T21:27:53] Filtering end-to-end fusions with low support (remaining=1853)
[2026-06-04T21:27:54] Filtering fusions with no coverage around the breakpoints (remaining=1809)
[2026-06-04T21:27:55] Indexing gene sequences 
[2026-06-04T21:28:01] Filtering genes with >=30% identity (remaining=593)
[2026-06-04T21:28:05] Re-aligning chimeric reads to filter fusions with >=80% mis-mappers (remaining=363)
[2026-06-04T21:28:13] Selecting best breakpoints from genes with multiple breakpoints (remaining=274)
[2026-06-04T21:28:14] Searching for additional isoforms (remaining=391)
[2026-06-04T21:28:15] Assigning confidence scores to events 
[2026-06-04T21:28:18] Loading protein domains from 'protein_domains_hg38_GRCh38_v2.4.0.gff3'
[2026-06-04T21:28:19] Writing fusions to file '659_dHy-T1-TRNA-1_B23MHV2LT4_1.arriba.fusions.tsv' 
[2026-06-04T21:28:22] Writing discarded fusions to file '659_dHy-T1-TRNA-1_B23MHV2LT4_1.arriba.fusions.discarded.tsv'
[2026-06-04T21:28:47] Freeing resources
[2026-06-04T21:29:04] Done (elapsed time=00:11:19, CPU time=00:11:18, peak memory=15.5gb)