FastQCFastQC Report
Mon 8 Jun 2026
659_Kh-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_Kh-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433034
Total Bases518.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTAGTGACATCTAGT2121076.178412447997894TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTAGTGACATCTGGT338660.9864743547544244TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTAGTGACATCTAGG66860.19475484367472037TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTAGTGACATCTGGG62330.18155951849005866TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTAGTGACATCTAGTA43420.12647704625121683TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)