FastQCFastQC Report
Mon 8 Jun 2026
659_R1-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_R1-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500047
Total Bases75.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTAGT28774857.544190846060474TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTGGT8526817.051997112271447TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTGGG104572.0912034268778736TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTAGG75841.5166574342011852TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTAGTA45210.9041150131887603TruSeq Adapter, Index 13 (97% over 37bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG26810.5361496019374179No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA24420.4883540947150967No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC21130.4225602793337426No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTAGTATG17540.35076702789937747TruSeq Adapter, Index 13 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTAGTG15070.30137167106292007TruSeq Adapter, Index 13 (97% over 37bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC13820.2763740208420408No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCGAGT10800.2159796979083966TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTGGTA10120.20238097618823833TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTGGTG9750.19498167172285807TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCGGGT8760.1751835327479217TruSeq Adapter, Index 13 (97% over 38bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA7640.1527856381500139No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATGTAGT7630.15258565694824686TruSeq Adapter, Index 13 (97% over 38bp)
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGA7130.14258659685989516No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATATAGT6960.1391869164298556TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATGTGGT6900.13798702921925338TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATTTAGT6450.12898787513973686TruSeq Adapter, Index 13 (97% over 38bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAGATCGGAAGAGCAC6450.12898787513973686No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC6140.1227884578849588No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTAGTGTG5600.11198947298953897TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATTTGGT5450.10898975496303348TruSeq Adapter, Index 13 (97% over 38bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCAGATCGGAAGAGCA5200.10399022491885763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)