FastQCFastQC Report
Mon 8 Jun 2026
659_b6o-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_b6o-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437890
Total Bases66.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTAGT18382341.97926419877138TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGGT13898631.739934686793486TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGGG261005.960401013953276TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTAGG115122.628970746077782TruSeq Adapter, Index 10 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTAGTA27990.6392016259791272TruSeq Adapter, Index 10 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGGTA17370.3966749640320628TruSeq Adapter, Index 10 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGGTG13610.31080865057434515TruSeq Adapter, Index 10 (97% over 36bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG13150.30030372924707116No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCGGGT12100.27632510447829367TruSeq Adapter, Index 10 (97% over 37bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC11200.25577199753362717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCGAGT10910.24914932974034573TruSeq Adapter, Index 10 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTAGTATG10150.2317933727648496TruSeq Adapter, Index 10 (97% over 34bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA10020.22882459065062002No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATGTGGT9510.21717783004864236TruSeq Adapter, Index 10 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTAGTG8950.204389230171961TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATGTAGT8310.1897736874557537TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATATAGT7790.1778985589988353TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATATGGT7430.16967731622096874TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATTTGGT6550.14958094498618374TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCGGGG6550.14958094498618374TruSeq Adapter, Index 10 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGGTATG6000.13702071296444313TruSeq Adapter, Index 10 (97% over 34bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC5850.13359519514033205No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATTTAGT5420.12377537737788029TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCGAGG5390.12309027381305808TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATGTGGG5380.12286190595811733TruSeq Adapter, Index 10 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGGGG5060.11555413460001371TruSeq Adapter, Index 10 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGGTGTG4720.1077896275320286TruSeq Adapter, Index 10 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)