FastQCFastQC Report
Mon 8 Jun 2026
659_buj-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_buj-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319430
Total Bases48.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGT15665949.04329587076981TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTGGT5235916.391384653914788TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTGGG222646.96991516138121TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGG200366.272422753028832TruSeq Adapter, Index 9 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGTG16110.5043358482296592TruSeq Adapter, Index 9 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA11340.3550073568543969No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT10640.3330933224806687No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGTA9890.3096139999373885TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC7550.2363585136023542No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTGGTG7430.23260182199542934TruSeq Adapter, Index 9 (97% over 36bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC7040.22039257427292364No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATATAGT6670.20880944181823877TruSeq Adapter, Index 9 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGTGTG6510.20380051967567228TruSeq Adapter, Index 9 (97% over 34bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC5990.18752152271233133No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG5960.18658234981060012No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCGAGT5940.18595623454277932TruSeq Adapter, Index 9 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGTATG5220.1634160849012303TruSeq Adapter, Index 9 (97% over 34bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG4890.153085182982187No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCGGGT4450.13931064709012927TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCGAGG4210.13179726387627963TruSeq Adapter, Index 9 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTGGGG3700.11583132454684908TruSeq Adapter, Index 9 (97% over 36bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGCAGATCGGAAGAGCA3660.11457909401120746No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATATGGT3570.11176157530601383TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATGTAGT3500.10957017186864103TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATATAGG3440.1076918260651786TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCGGGG3440.1076918260651786TruSeq Adapter, Index 9 (97% over 37bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAGATCGGAAGAGCAC3420.1070657107973578No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA3390.1061265378956266No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGT3230.10111761575306014TruSeq Adapter, Index 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)