FastQCFastQC Report
Mon 8 Jun 2026
659_cAa-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_cAa-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332597
Total Bases50.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC73

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTAGT20828362.623234725508645TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTGGT7261321.832127168916134TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTAGTA45201.3590020354964114TruSeq Adapter, Index 2 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTAGTATG19380.5826871559274438TruSeq Adapter, Index 2 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTGGTA15100.4540028923892879TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTGGG11280.33914918053981247TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCGAGT10410.31299139799817793TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATGTAGT8810.2648851312549422TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATTTAGT8370.2516559079005523TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCGGGT7840.23572070704185547TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATGTGGT7470.22459613285748217TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATTTGGT7330.22038683451744903TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATATAGT5930.1782938511171177TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTGGTATG5710.17167923943992278TruSeq Adapter, Index 2 (96% over 33bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTAGTA4530.13620086771678638TruSeq Adapter, Index 23 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAATGTATATCTAGT4500.1352988752153507TruSeq Adapter, Index 2 (97% over 36bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG4270.12838359937101057No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTAGG4270.12838359937101057TruSeq Adapter, Index 2 (97% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTAGTAT4030.1211676593595252TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATATGGT3970.11936367435665385TruSeq Adapter, Index 2 (97% over 36bp)
GATCGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTAGTA3800.11425238351518503TruSeq Adapter, Index 2 (96% over 31bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTAGT3510.10553312266797356TruSeq Adapter, Index 23 (97% over 36bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA3410.10252648099652131No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC3370.10132382432794043No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)