FastQCFastQC Report
Mon 8 Jun 2026
659_cuO-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_cuO-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192558
Total Bases29 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTAGT7990541.49658804100583TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTGGT4718924.506382492547697TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTGGG147557.6626263255746325TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTAGG105655.4866585652115205TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTAGTA9550.49595446566748724TruSeq Adapter, Index 1 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA7910.41078532182511246No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT6430.3339253627478474No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTAGTATG6090.3162683451219892TruSeq Adapter, Index 1 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATATAGT6030.31315240083507306TruSeq Adapter, Index 1 (97% over 36bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4870.2529108112880275No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTGGTA4840.2513528391445694TruSeq Adapter, Index 1 (97% over 35bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG4780.2482368948576533No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATATGGT4720.24512095057073713TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTGGTG3900.20253637864954974TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCGAGT3860.2004590824582723TruSeq Adapter, Index 1 (97% over 36bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC3390.17605085221076247No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTAGTG3340.17345423197166568TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATATAGG3250.16878031554129147TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCGGGT3230.16774166744565275TruSeq Adapter, Index 1 (97% over 36bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC3010.15631653839362686No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG2910.15112329791543327No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCGGGG2830.14696870553287839TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATATGGG2790.14489140934160097TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATGTAGT2770.14385276124596225TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCGAGG2680.13917884481558804TruSeq Adapter, Index 1 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTGGTATG2680.13917884481558804TruSeq Adapter, Index 1 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATGTGGT2360.12256047528536856TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTGGGG2360.12256047528536856TruSeq Adapter, Index 1 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATCTAGT2150.11165467028116205TruSeq Adapter, Index 3 (97% over 35bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGCAGATCGGAAGAGCA2070.10750007789860719No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTACCGGCATGTGGG2040.10594210575514909TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)