FastQCFastQC Report
Mon 8 Jun 2026
659_d0M-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_d0M-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395877
Total Bases59.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC73

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTAGT22586357.053832377228275TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTGGT9443323.854126407949945TruSeq Adapter, Index 2 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTAGTA44071.1132245621746149TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTGGG23860.6027124586677175TruSeq Adapter, Index 2 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTGGTA19390.48979859905980905TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTAGTATG17770.44887679759117105TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCGAGT11990.302871851610475TruSeq Adapter, Index 2 (97% over 38bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG11560.29200989196139204No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA11060.2793797063229235No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCGGGT10210.2579083907375271TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTAGG10090.25487714618429463TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATGTAGT9990.2523511090566009TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATGTGGT9330.2356792640138225TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATTTAGT8510.2149657595667341TruSeq Adapter, Index 2 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTGGTATG7780.19652568853457006TruSeq Adapter, Index 2 (97% over 35bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC7670.193747047694107No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATTTGGT7470.1886949734387196TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATATAGT7140.1803590509173304TruSeq Adapter, Index 2 (97% over 38bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6120.15459347221485462No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC5620.1419632865763861No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATATGGT5260.13286955291668878TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTAGTA5200.13135393064007253TruSeq Adapter, Index 7 (96% over 30bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4560.11518729302283286No Hit
GATCGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGTGTATCTAGTA4040.10205189995882559TruSeq Adapter, Index 2 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)