[W::hts_idx_load3] The index file is older than the data file: 659_bGc-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13994956) splicing reads. 253517 splicing junctions. 188256 known splicing junctions. 65261 novel splicing junctions.
sampling 10% (27989913) splicing reads. 304974 splicing junctions. 200848 known splicing junctions. 104126 novel splicing junctions.
sampling 15% (41984870) splicing reads. 340836 splicing junctions. 207560 known splicing junctions. 133276 novel splicing junctions.
sampling 20% (55979827) splicing reads. 368730 splicing junctions. 211932 known splicing junctions. 156798 novel splicing junctions.
sampling 25% (69974784) splicing reads. 391261 splicing junctions. 215128 known splicing junctions. 176133 novel splicing junctions.
sampling 30% (83969741) splicing reads. 410176 splicing junctions. 217570 known splicing junctions. 192606 novel splicing junctions.
sampling 35% (97964698) splicing reads. 426180 splicing junctions. 219555 known splicing junctions. 206625 novel splicing junctions.
sampling 40% (111959655) splicing reads. 439818 splicing junctions. 221127 known splicing junctions. 218691 novel splicing junctions.
sampling 45% (125954612) splicing reads. 451634 splicing junctions. 222407 known splicing junctions. 229227 novel splicing junctions.
sampling 50% (139949569) splicing reads. 461840 splicing junctions. 223426 known splicing junctions. 238414 novel splicing junctions.
sampling 55% (153944525) splicing reads. 470721 splicing junctions. 224295 known splicing junctions. 246426 novel splicing junctions.
sampling 60% (167939482) splicing reads. 478421 splicing junctions. 225064 known splicing junctions. 253357 novel splicing junctions.
sampling 65% (181934439) splicing reads. 485253 splicing junctions. 225739 known splicing junctions. 259514 novel splicing junctions.
sampling 70% (195929396) splicing reads. 490991 splicing junctions. 226301 known splicing junctions. 264690 novel splicing junctions.
sampling 75% (209924353) splicing reads. 495939 splicing junctions. 226749 known splicing junctions. 269190 novel splicing junctions.
sampling 80% (223919310) splicing reads. 500244 splicing junctions. 227175 known splicing junctions. 273069 novel splicing junctions.
sampling 85% (237914267) splicing reads. 503774 splicing junctions. 227525 known splicing junctions. 276249 novel splicing junctions.
sampling 90% (251909224) splicing reads. 506653 splicing junctions. 227782 known splicing junctions. 278871 novel splicing junctions.
sampling 95% (265904181) splicing reads. 508839 splicing junctions. 227983 known splicing junctions. 280856 novel splicing junctions.
sampling 100% (279899138) splicing reads. 510414 splicing junctions. 228142 known splicing junctions. 282272 novel splicing junctions.