[2026-06-08T07:45:15] Launching Arriba 2.4.0
[2026-06-08T07:45:15] Loading assembly from 'ref_genome.fa' 
[2026-06-08T07:45:27] Loading annotation from 'ref_annot.gtf' 
[2026-06-08T07:45:31] Reading chimeric alignments from '659_cGW-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam' (total=4899819)
[2026-06-08T07:48:23] Marking multi-mapping alignments (marked=3371988)
[2026-06-08T07:48:25] Detecting strandedness (reverse)
[2026-06-08T07:48:25] Assigning strands to alignments 
[2026-06-08T07:48:27] Annotating alignments 
[2026-06-08T07:48:52] Filtering duplicates (remaining=1414313)
[2026-06-08T07:48:57] Filtering mates which do not map to interesting contigs (1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y AC_* NC_*) (remaining=1175174)
[2026-06-08T07:48:58] Filtering mates which only map to viral contigs (AC_* NC_*) (remaining=1175174)
[2026-06-08T07:49:00] Filtering viral contigs with expression lower than the top 5 (remaining=1175174)
[2026-06-08T07:49:02] Filtering viral contigs with less than 5% coverage (remaining=1175174)
[2026-06-08T07:49:04] Estimating fragment length (mate gap mean=-84.4229, mate gap stddev=28.9935, read length mean=124.79)
[2026-06-08T07:49:04] Filtering read-through fragments with a distance <=10000bp (remaining=1123989)
[2026-06-08T07:49:05] Filtering inconsistently clipped mates (remaining=1096024)
[2026-06-08T07:49:07] Filtering breakpoints adjacent to homopolymers >=6nt (remaining=1053514)
[2026-06-08T07:49:09] Filtering fragments with small insert size (remaining=1052936)
[2026-06-08T07:49:10] Filtering alignments with long gaps (remaining=1052936)
[2026-06-08T07:49:11] Filtering fragments with both mates in the same gene (remaining=1052615)
[2026-06-08T07:49:13] Filtering fusions arising from hairpin structures (remaining=992294)
[2026-06-08T07:49:14] Filtering reads with a mismatch p-value <=0.01 (remaining=419209)
[2026-06-08T07:49:19] Filtering reads with low entropy (k-mer content >=60%) (remaining=148469)
[2026-06-08T07:49:23] Finding fusions and counting supporting reads (total=124916)
[2026-06-08T07:49:32] Merging adjacent fusion breakpoints (remaining=124116)
[2026-06-08T07:49:33] Filtering multi-mapping fusions by alignment score and read support (remaining=66320)
[2026-06-08T07:49:54] Estimating expected number of fusions by random chance (e-value) 
[2026-06-08T07:49:56] Filtering fusions with both breakpoints in adjacent non-coding/intergenic regions (remaining=65760)
[2026-06-08T07:49:56] Filtering intragenic fusions with both breakpoints in exonic regions (remaining=35909)
[2026-06-08T07:49:57] Filtering fusions with <2 supporting reads (remaining=3248)
[2026-06-08T07:49:57] Filtering fusions with an e-value >=0.3 (remaining=831)
[2026-06-08T07:49:57] Searching for internal tandem duplications <=100bp with >=10 supporting reads and >=7% allele fraction (remaining=847)
[2026-06-08T07:49:59] Filtering fusions with both breakpoints in intronic/intergenic regions (remaining=799)
[2026-06-08T07:49:59] Searching for known fusions in 'known_fusions_hg38_GRCh38_v2.4.0.tsv.gz' (remaining=799)
[2026-06-08T07:50:00] Filtering in vitro-generated fusions between genes with an expression above the 99.8% quantile (remaining=779)
[2026-06-08T07:50:03] Searching for fusions with spliced split reads (remaining=829)
[2026-06-08T07:50:06] Selecting best breakpoints from genes with multiple breakpoints (remaining=675)
[2026-06-08T07:50:06] Filtering read-through fusions with breakpoints near the gene boundary (remaining=669)
[2026-06-08T07:50:06] Searching for fusions with >=4 spliced events (remaining=701)
[2026-06-08T07:50:07] Filtering blacklisted fusions in 'blacklist_hg38_GRCh38_v2.4.0.tsv.gz' (remaining=185)
[2026-06-08T07:50:27] Filtering fusions with anchors <=23nt (remaining=152)
[2026-06-08T07:50:27] Filtering end-to-end fusions with low support (remaining=149)
[2026-06-08T07:50:27] Filtering fusions with no coverage around the breakpoints (remaining=144)
[2026-06-08T07:50:28] Indexing gene sequences 
[2026-06-08T07:50:30] Filtering genes with >=30% identity (remaining=57)
[2026-06-08T07:50:30] Re-aligning chimeric reads to filter fusions with >=80% mis-mappers (remaining=22)
[2026-06-08T07:50:31] Selecting best breakpoints from genes with multiple breakpoints (remaining=19)
[2026-06-08T07:50:31] Searching for additional isoforms (remaining=23)
[2026-06-08T07:50:32] Assigning confidence scores to events 
[2026-06-08T07:50:33] Loading protein domains from 'protein_domains_hg38_GRCh38_v2.4.0.gff3'
[2026-06-08T07:50:33] Writing fusions to file '659_cGW-T1-TRNA-1_B23MHV2LT4_1.arriba.fusions.tsv' 
[2026-06-08T07:50:33] Writing discarded fusions to file '659_cGW-T1-TRNA-1_B23MHV2LT4_1.arriba.fusions.discarded.tsv'
[2026-06-08T07:50:45] Freeing resources
[2026-06-08T07:50:55] Done (elapsed time=00:05:40, CPU time=00:05:40, peak memory=9.11gb)