[W::hts_idx_load3] The index file is older than the data file: 659_bR4-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	292349702
Known Splicing Events:	288567806
Partial Novel Splicing Events:	2090874
Novel Splicing Events:	1578852
Filtered Splicing Events:	112170

Total splicing  Junctions:	692591
Known Splicing Junctions:	312597
Partial Novel Splicing Junctions:	219099
Novel Splicing Junctions:	160895

===================================================================
Create BED file ...
Create Interact file ...