[2026-06-08T08:37:50] Launching Arriba 2.4.0
[2026-06-08T08:37:50] Loading assembly from 'ref_genome.fa' 
[2026-06-08T08:38:05] Loading annotation from 'ref_annot.gtf' 
[2026-06-08T08:38:10] Reading chimeric alignments from '659_epP-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam' (total=11570812)
[2026-06-08T08:48:47] Marking multi-mapping alignments (marked=7321168)
[2026-06-08T08:48:54] Detecting strandedness (reverse)
[2026-06-08T08:48:54] Assigning strands to alignments 
[2026-06-08T08:48:58] Annotating alignments 
[2026-06-08T08:49:53] Filtering duplicates (remaining=6669352)
[2026-06-08T08:50:08] Filtering mates which do not map to interesting contigs (1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y AC_* NC_*) (remaining=6312156)
[2026-06-08T08:50:12] Filtering mates which only map to viral contigs (AC_* NC_*) (remaining=6312156)
[2026-06-08T08:50:15] Filtering viral contigs with expression lower than the top 5 (remaining=6312156)
[2026-06-08T08:50:22] Filtering viral contigs with less than 5% coverage (remaining=6312156)
[2026-06-08T08:50:26] Estimating fragment length (mate gap mean=-89.026, mate gap stddev=29.1443, read length mean=136.897)
[2026-06-08T08:50:26] Filtering read-through fragments with a distance <=10000bp (remaining=5776771)
[2026-06-08T08:50:30] Filtering inconsistently clipped mates (remaining=5673729)
[2026-06-08T08:50:33] Filtering breakpoints adjacent to homopolymers >=6nt (remaining=5442189)
[2026-06-08T08:50:40] Filtering fragments with small insert size (remaining=5441343)
[2026-06-08T08:50:43] Filtering alignments with long gaps (remaining=5441343)
[2026-06-08T08:50:46] Filtering fragments with both mates in the same gene (remaining=5440435)
[2026-06-08T08:50:51] Filtering fusions arising from hairpin structures (remaining=5056864)
[2026-06-08T08:50:55] Filtering reads with a mismatch p-value <=0.01 (remaining=2711901)
[2026-06-08T08:51:16] Filtering reads with low entropy (k-mer content >=60%) (remaining=1218128)
[2026-06-08T08:51:34] Finding fusions and counting supporting reads (total=909144)
[2026-06-08T08:52:04] Merging adjacent fusion breakpoints (remaining=899756)
[2026-06-08T08:52:07] Filtering multi-mapping fusions by alignment score and read support (remaining=544399)
[2026-06-08T08:53:13] Estimating expected number of fusions by random chance (e-value) 
[2026-06-08T08:53:27] Filtering fusions with both breakpoints in adjacent non-coding/intergenic regions (remaining=540802)
[2026-06-08T08:53:28] Filtering intragenic fusions with both breakpoints in exonic regions (remaining=280436)
[2026-06-08T08:53:30] Filtering fusions with <2 supporting reads (remaining=40227)
[2026-06-08T08:53:31] Filtering fusions with an e-value >=0.3 (remaining=14858)
[2026-06-08T08:53:32] Searching for internal tandem duplications <=100bp with >=10 supporting reads and >=7% allele fraction (remaining=14919)
[2026-06-08T08:53:38] Filtering fusions with both breakpoints in intronic/intergenic regions (remaining=14743)
[2026-06-08T08:53:39] Searching for known fusions in 'known_fusions_hg38_GRCh38_v2.4.0.tsv.gz' (remaining=14744)
[2026-06-08T08:53:41] Filtering in vitro-generated fusions between genes with an expression above the 99.8% quantile (remaining=7537)
[2026-06-08T08:53:49] Searching for fusions with spliced split reads (remaining=7562)
[2026-06-08T08:53:57] Selecting best breakpoints from genes with multiple breakpoints (remaining=4547)
[2026-06-08T08:53:59] Filtering read-through fusions with breakpoints near the gene boundary (remaining=4502)
[2026-06-08T08:54:00] Searching for fusions with >=4 spliced events (remaining=5452)
[2026-06-08T08:54:02] Filtering blacklisted fusions in 'blacklist_hg38_GRCh38_v2.4.0.tsv.gz' (remaining=2090)
[2026-06-08T08:54:31] Filtering fusions with anchors <=23nt (remaining=1685)
[2026-06-08T08:54:32] Filtering end-to-end fusions with low support (remaining=1608)
[2026-06-08T08:54:33] Filtering fusions with no coverage around the breakpoints (remaining=1541)
[2026-06-08T08:54:34] Indexing gene sequences 
[2026-06-08T08:54:50] Filtering genes with >=30% identity (remaining=445)
[2026-06-08T08:54:54] Re-aligning chimeric reads to filter fusions with >=80% mis-mappers (remaining=165)
[2026-06-08T08:54:58] Selecting best breakpoints from genes with multiple breakpoints (remaining=151)
[2026-06-08T08:55:00] Searching for additional isoforms (remaining=228)
[2026-06-08T08:55:02] Assigning confidence scores to events 
[2026-06-08T08:55:09] Loading protein domains from 'protein_domains_hg38_GRCh38_v2.4.0.gff3'
[2026-06-08T08:55:10] Writing fusions to file '659_epP-T1-TRNA-1_B23MHV2LT4_1.arriba.fusions.tsv' 
[2026-06-08T08:55:13] Writing discarded fusions to file '659_epP-T1-TRNA-1_B23MHV2LT4_1.arriba.fusions.discarded.tsv'
[2026-06-08T08:56:15] Freeing resources
[2026-06-08T08:56:59] Done (elapsed time=00:19:09, CPU time=00:19:07, peak memory=16.5gb)