[W::hts_idx_load3] The index file is older than the data file: 659_bTq-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	330602319
Known Splicing Events:	327091937
Partial Novel Splicing Events:	1909492
Novel Splicing Events:	1519456
Filtered Splicing Events:	81434

Total splicing  Junctions:	658747
Known Splicing Junctions:	297915
Partial Novel Splicing Junctions:	209894
Novel Splicing Junctions:	150938

===================================================================
Create BED file ...
Create Interact file ...