[W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	212861861
Known Splicing Events:	210809282
Partial Novel Splicing Events:	1241902
Novel Splicing Events:	723582
Filtered Splicing Events:	87095

Total splicing  Junctions:	346509
Known Splicing Junctions:	224005
Partial Novel Splicing Junctions:	73592
Novel Splicing Junctions:	48912

===================================================================
Create BED file ...
Create Interact file ...