[W::hts_idx_load3] The index file is older than the data file: 659_bhK-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	358339020
Known Splicing Events:	355480936
Partial Novel Splicing Events:	1299610
Novel Splicing Events:	1469345
Filtered Splicing Events:	89129

Total splicing  Junctions:	576565
Known Splicing Junctions:	281454
Partial Novel Splicing Junctions:	162491
Novel Splicing Junctions:	132620

===================================================================
Create BED file ...
Create Interact file ...