Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ef/f65ccd10cd4cb1d545dec86bc53fb5/.command.sh
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/32/dbf240306ccc650510191477cddb38/659_cGW-T1-TRNA-1_B23MHV2LT4_1.Chimeric.out.junction
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ff/1b64228c77bbad4660d4533eb8a98b/659_cGW-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ef/f65ccd10cd4cb1d545dec86bc53fb5/.command.run
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/32/dbf240306ccc650510191477cddb38/659_cGW-T1-TRNA-1_B23MHV2LT4_1.SJ.out.tab
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/32/dbf240306ccc650510191477cddb38/659_cGW-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (7 inputs)
07:49:03 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes
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[4210000], rate=15787500.00/min 07:49:25 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer.introns.prelim
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns: identified 1528 cancer introns
07:49:25 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer.introns.prelim
07:49:25 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/prelim_introns.ok
07:49:25 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer.introns.prelim --min_total_reads 5 > 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer.introns
07:49:25 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer.introns.prelim --min_total_reads 5 > 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer.introns
07:49:25 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/introns_filtered.ok
07:49:25 : INFO : -found 959 cancer introns
07:49:25 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns --cancer_introns 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed
07:49:26 : INFO : Creating the BED File.
07:49:29 : INFO : Saving Bed File as 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed
07:49:29 : INFO : Execution Time = 0.06 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns --cancer_introns 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed
07:49:29 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/intron_igv_bed.ok
07:49:29 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed --bam 659_cGW-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam --output_prefix 659_cGW-T1-TRNA-1_B23MHV2LT4_1
[W::hts_idx_load3] The index file is older than the data file: 659_cGW-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
07:54:37 : INFO : Execution Time = 5.14 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed --bam 659_cGW-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam --output_prefix 659_cGW-T1-TRNA-1_B23MHV2LT4_1
07:54:37 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/reads_alignments_extracted.ok
07:54:37 : INFO : Running: samtools sort -o 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.sorted.bam 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.bam
07:54:37 : INFO : Execution Time = 0.00 minutes. CMD: samtools sort -o 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.sorted.bam 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.bam
07:54:37 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/sort_cancer_intron_reads.ok
07:54:37 : INFO : Running: samtools sort -o 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.bam 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.bam
[bam_sort_core] merging from 23 files and 1 in-memory blocks...
08:00:01 : INFO : Execution Time = 5.39 minutes. CMD: samtools sort -o 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.bam 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.bam
08:00:01 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/sort_gene_reads.ok
08:00:01 : INFO : Running: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmp --keep_secondary 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.bam
Done with chr chr1.
Done with chr chr2.
Done with chr chr3.
Done with chr chr4.
Done with chr chr5.
Done with chr chr6.
Done with chr chr7.
Done with chr chr8.
Done with chr chr9.
Done with chr chr10.
Done with chr chr11.
Done with chr chr12.
Done with chr chr13.
Done with chr chr14.
Done with chr chr15.
Done with chr chr16.
Done with chr chr17.
Done with chr chr18.
Done with chr chr19.
Done with chr chr20.
Done with chr chr21.
Done with chr chr22.
08:01:40 : INFO : Execution Time = 1.66 minutes. CMD: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmp --keep_secondary 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.bam
08:01:40 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmp.ok
08:01:40 : INFO : Running: samtools sort -o 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmp
08:01:50 : INFO : Execution Time = 0.16 minutes. CMD: samtools sort -o 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmp
08:01:50 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmpsorted.ok
08:01:50 : INFO : Running: samtools index 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam
08:01:51 : INFO : Execution Time = 0.02 minutes. CMD: samtools index 659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam
08:01:51 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/659_cGW-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.indexed.ok
08:01:51 : INFO : Running: samtools index 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.sorted.bam
08:01:51 : INFO : Execution Time = 0.00 minutes. CMD: samtools index 659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.sorted.bam
08:01:51 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/659_cGW-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.sorted.bam.indexed.ok
08:01:51 : INFO : Running: create_report 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output 659_cGW-T1-TRNA-1_B23MHV2LT4_1.ctat-splicing.igv.html --track-config 659_cGW-T1-TRNA-1_B23MHV2LT4_1.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: 659_cGW-T1-TRNA-1_B23MHV2LT4_1'
08:03:14 : INFO : Execution Time = 1.38 minutes. CMD: create_report 659_cGW-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output 659_cGW-T1-TRNA-1_B23MHV2LT4_1.ctat-splicing.igv.html --track-config 659_cGW-T1-TRNA-1_B23MHV2LT4_1.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: 659_cGW-T1-TRNA-1_B23MHV2LT4_1'
08:03:14 : INFO : Running: touch /tmp/nxf.fyM64T1OjP/659_cGW-T1-TRNA-1_B23MHV2LT4_1.chckpts/igv_create_html.ok
08:03:14 : INFO : done.